FlyBase Allele Report: Dmel\Atg1[HMS02750]

General Information

Symbol

Dmel\Atg1^HMS02750

Species

D. melanogaster

Name

FlyBase ID

FBal0293390

Feature type

allele

Associated gene

Dmel\Atg1

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS02750}

Also Known As

UAS-atg1^RNAi, atg1^RNAi, Atg1 RNAi, HMS02750, Atg1RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS02750}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS02750

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^Δ166, Vap33^ALS8

(Thulasidharan et al., 2024)

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 2

modeled by Chchd2^S81L.UAS

(Baek et al., 2021)

ameliorates syndrome

modeled by RpS23^R67K

(Recasens-Alvarez et al., 2021)

ameliorates cancer

modeled by Raf^F22.hs

(Zhao et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | P-stage, with Scer\GAL4^ppk.PU

(Tan et al., 2024)

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Sopko et al., 2014)

increased cell size, with Scer\GAL4^NP0001

(Wen et al., 2017)

short lived, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adulthood-only expression of Atg1^HMS02750 under the combined control of Scer\GAL4^tub.PU and Gal80[ts] leads to intestinal barrier disfunction (as shown by the abnormal permeability to a non-absorbable food dye), leads to the failure to induce autophagy (assessed by the lysosome and autophagosome marker, Lysotracker) in the adult fat body in response to starvation, and leads to a decreased lifespan, as compared to controls. Constitutive or adulthood-only expression under the control of Scer\GAL4^{Myo31DF-NP0001} alone or in combination with Gal80[ts], respectively, leads to pronounced defects in the midgut, namely a severe increase in cell size and and abnormal epithelium morphology, as compared to controls.

(Wen et al., 2017)

Females heterozygous for Atg1^HMS02750 and MTD-Gal4 (consists of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) crossed to Atg1^HMS02750 males results in: 45% hatching; maternal effect.

(Sopko et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Atg1^HMS02750, Scer\GAL4^ppk.PU has abnormal neuroanatomy | P-stage phenotype, enhanceable by Diap1^UAS.cHa, Scer\GAL4^ppk.PU

(Tan et al., 2024)

Atg1^HMS02750, Scer\GAL4^ppk.PU has abnormal neuroanatomy | P-stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ppk.PU

(Tan et al., 2024)

Suppressed by

Statement

Reference

Atg1^HMS02750, Scer\GAL4^ppk.PU has abnormal neuroanatomy | P-stage phenotype, suppressible | partially by ref(2)P^HMS00938, Scer\GAL4^ppk.PU

(Tan et al., 2024)

NOT suppressed by

Statement

Reference

Atg1^HMS02750, Scer\GAL4^NP0001 has increased cell size phenotype, non-suppressible by Patj^UAS.Tag:MYC, Scer\GAL4^NP0001

(Wen et al., 2017)

Enhancer of

Statement

Reference

Atg1^HMS02750, Scer\GAL4^{elav.Switch.PO} is an enhancer of short lived | RU486 conditional phenotype of Scer\GAL4^{elav.Switch.PO}, Zzzz\GGGGCC^36.UAS

(Xu et al., 2023)

Atg1^HMS02750/Scer\GAL4^rn-GAL4-5 is an enhancer of increased cell death | larval stage | somatic clone phenotype of RpS23^R67K

(Recasens-Alvarez et al., 2021)

NOT Enhancer of

Statement

Reference

Atg1^HMS02750/bumpel^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^{elav.Switch.PO} is a non-enhancer of short lived | RU486 conditional phenotype of Scer\GAL4^{elav.Switch.PO}, Zzzz\GGGGCC^36.UAS

(Xu et al., 2023)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of decreased cell number | adult stage phenotype of Phb2^GD8538, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of decreased cell number | adult stage phenotype of Phb2^HMS02001, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^esg.PU is a non-enhancer of decreased cell number | adult stage | somatic clone phenotype of dind^Δ66

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^esg.PU is a non-enhancer of decreased occurrence of cell division | adult stage | somatic clone phenotype of dind^Δ9

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of decreased cell number | adult stage phenotype of Scer\GAL4^esg.PU, dind^NIG.7705R

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of decreased cell number | adult stage phenotype of Scer\GAL4^esg.PU, dind^{RNAi.UAS.RNAi}

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^Act5C.PI is a non-enhancer of decreased cell number | adult stage | somatic clone phenotype of Phb1²⁰

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of decreased cell number | adult stage phenotype of Phb1^HMS00399, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of decreased cell number | adult stage phenotype of Phb1^HMS00702, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^Act5C.PI is a non-enhancer of decreased cell number | adult stage | somatic clone phenotype of Phb2^Δ

(Zhao et al., 2022)

NOT Suppressor of

Statement

Reference

Atg1^HMS02750/bumpel^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^{elav.Switch.PO} is a non-suppressor of short lived | RU486 conditional phenotype of Scer\GAL4^{elav.Switch.PO}, Zzzz\GGGGCC^36.UAS

(Xu et al., 2023)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of decreased cell number | adult stage phenotype of Phb2^GD8538, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of decreased cell number | adult stage phenotype of Phb2^HMS02001, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^esg.PU is a non-suppressor of decreased cell number | somatic clone | adult stage phenotype of dind^Δ66

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^esg.PU is a non-suppressor of decreased occurrence of cell division | somatic clone | adult stage phenotype of dind^Δ9

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of decreased cell number | adult stage phenotype of Scer\GAL4^esg.PU, dind^NIG.7705R

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of decreased cell number | adult stage phenotype of Scer\GAL4^esg.PU, dind^{RNAi.UAS.RNAi}

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^Act5C.PI is a non-suppressor of decreased cell number | somatic clone | adult stage phenotype of Phb1²⁰

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of decreased cell number | adult stage phenotype of Phb1^HMS00399, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of decreased cell number | adult stage phenotype of Phb1^HMS00702, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^Act5C.PI is a non-suppressor of decreased cell number | somatic clone | adult stage phenotype of Phb2^Δ

(Zhao et al., 2022)

Phenotype Manifest In

Enhanced by

Statement

Reference

Atg1^HMS02750, Scer\GAL4^ppk.PU has larval dorsal multidendritic neuron ddaC | P-stage phenotype, enhanceable by Diap1^UAS.cHa, Scer\GAL4^ppk.PU

(Tan et al., 2024)

Atg1^HMS02750, Scer\GAL4^ppk.PU has dendrite | P-stage phenotype, enhanceable by Diap1^UAS.cHa, Scer\GAL4^ppk.PU

(Tan et al., 2024)

Atg1^HMS02750, Scer\GAL4^ppk.PU has larval dorsal multidendritic neuron ddaC | P-stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ppk.PU

(Tan et al., 2024)

Atg1^HMS02750, Scer\GAL4^ppk.PU has dendrite | P-stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ppk.PU

(Tan et al., 2024)

Suppressed by

Statement

Reference

Atg1^HMS02750, Scer\GAL4^ppk.PU has larval dorsal multidendritic neuron ddaC | P-stage phenotype, suppressible | partially by ref(2)P^HMS00938, Scer\GAL4^ppk.PU

(Tan et al., 2024)

Atg1^HMS02750, Scer\GAL4^ppk.PU has dendrite | P-stage phenotype, suppressible | partially by ref(2)P^HMS00938, Scer\GAL4^ppk.PU

(Tan et al., 2024)

NOT suppressed by

Statement

Reference

Atg1^HMS02750, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, non-suppressible by Patj^UAS.Tag:MYC, Scer\GAL4^NP0001

(Wen et al., 2017)

Enhancer of

Statement

Reference

Atg1^HMS02750/Scer\GAL4^rn-GAL4-5 is an enhancer of imaginal disc phenotype of RpS23^R67K

(Recasens-Alvarez et al., 2021)

NOT Enhancer of

Statement

Reference

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of adult posterior midgut epithelium phenotype of Phb2^GD8538, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of adult posterior midgut epithelium phenotype of Phb2^HMS02001, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^esg.PU is a non-enhancer of adult posterior midgut epithelium phenotype of dind^Δ66

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^esg.PU is a non-enhancer of adult posterior midgut epithelium | somatic clone phenotype of dind^Δ9

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of adult posterior midgut epithelium phenotype of Scer\GAL4^esg.PU, dind^NIG.7705R

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^Act5C.PI is a non-enhancer of adult posterior midgut epithelium | somatic clone phenotype of Phb1²⁰

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of adult posterior midgut epithelium phenotype of Scer\GAL4^esg.PU, dind^{RNAi.UAS.RNAi}

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-enhancer of adult posterior midgut epithelium phenotype of Phb1^HMS00399, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^esg.PU is a non-enhancer of adult posterior midgut epithelium phenotype of Phb1^HMS00702, Scer\GAL4^Act5C.PI

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^Act5C.PI is a non-enhancer of adult posterior midgut epithelium | somatic clone phenotype of Phb2^Δ

(Zhao et al., 2022)

NOT Suppressor of

Statement

Reference

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of adult posterior midgut epithelium phenotype of Phb2^GD8538, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of adult posterior midgut epithelium phenotype of Phb2^HMS02001, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^esg.PU is a non-suppressor of adult posterior midgut epithelium phenotype of dind^Δ66

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^esg.PU is a non-suppressor of adult posterior midgut epithelium | somatic clone phenotype of dind^Δ9

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of adult posterior midgut epithelium phenotype of Scer\GAL4^esg.PU, dind^NIG.7705R

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^Act5C.PI is a non-suppressor of adult posterior midgut epithelium | somatic clone phenotype of Phb1²⁰

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of adult posterior midgut epithelium phenotype of Scer\GAL4^esg.PU, dind^{RNAi.UAS.RNAi}

(Zhao et al., 2022)

Atg1^HMS02750, Scer\GAL4^esg.PU is a non-suppressor of adult posterior midgut epithelium phenotype of Phb1^HMS00399, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^esg.PU is a non-suppressor of adult posterior midgut epithelium phenotype of Phb1^HMS00702, Scer\GAL4^Act5C.PI

(Zhao et al., 2022)

Atg1^HMS02750/Scer\GAL4^Act5C.PI is a non-suppressor of adult posterior midgut epithelium | somatic clone phenotype of Phb2^Δ

(Zhao et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Patj^{Scer\UAS.T:Hsap\MYC} suppresses the increased cell size and abnormal epithelium morphology in the adult midgut resulting from the expression of Atg1^HMS02750 under the control of Scer\GAL4^{Myo31DF-NP0001}.

(Wen et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

44034