FlyBase Allele Report: Dmel\Src42A[HMS02755]

General Information

Symbol

Dmel\Src42A^HMS02755

Species

D. melanogaster

Name

FlyBase ID

FBal0293395

Feature type

allele

Associated gene

Dmel\Src42A

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS02755}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS02755}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS02755

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | conditional, with Scer\GAL4^NP5130

(Cordero et al., 2014)

abnormal stress response | adult stage, with Scer\GAL4^NP5130

(Cordero et al., 2014)

decreased occurrence of cell division | adult stage | conditional, with Scer\GAL4^NP5130

(Cordero et al., 2014)

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

(Sopko et al., 2014)

Phenotype Manifest In

adult midgut, with Scer\GAL4^NP5130

(Cordero et al., 2014)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Cordero et al., 2014)

intestinal stem cell of posterior adult midgut epithelium | conditional, with Scer\GAL4^NP5130

(Cordero et al., 2014)

Detailed Description

Statement

Reference

Scer\GAL4^esg-NP5130-mediated expression of Src42A^HMS02755 (restricted to adult stages by using P{tubP-GAL80^ts}) almost completely suppresses the intestinal stem cell proliferation (regeneration) response that is normally seen in midguts after feeding flies with pathogenic bacteria.

Unchallenged flies expressing Src42A^HMS02755 via Scer\GAL4^esg-NP5130 (restricted to adult stages by using P{tubP-GAL80^ts}) have thinner midguts. Expression of Src42A^HMS02755 in the intestinal epithelium (using the 'escargot flip out' system') impairs homeostatic self-renewal in the adult posterior midgut. Unlike controls, 14- and 30-day-old Src42A^HMS02755 MARCM clones in the adult midgut fail to progressively grow over time and even decrease their size.

(Cordero et al., 2014)

Females heterozygous for Src42A^HMS02755 and Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} crossed to Src42A^HMS02755 males results in: 65% hatching; non-maternal effect.

(Sopko et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Src42A^HMS02755, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF