Open Close
General Information
Symbol
Dmel\α-Cat1
Species
D. melanogaster
Name
FlyBase ID
FBal0294012
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:
A 2403bp deletion resulting from the imprecise excision of P{EP}GE30561 removes the first exon of α-Cat, including the translation start site, and much of the intergenic region between α-Cat and CG32230. (Reported R4 coordinates converted to R5)
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Imprecise excision of P{EP}GE30561 removes a 2403bp region between the insertion site and the α-Cat. This deletes the first exon of α-Cat, including the translation start site and much of the intergenic region between α-Cat and the predicted gene CG32230.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
α-Cat1 mutant embryos show anterior cuticle defects and frequently display holes, some also develop a completely dorsal open phenotype or dorsal holes. The mutant embryos have similar number of amnioserosa cells at the onset of dorsal closure but show an increase in the number of delamination events, this however has no effect on the velocity of progression of the leading edge during the dorsal closure.
α-Cat1/α-Cat1 mutants die at a late embryonic stage with severe defects in head morphogenesis. α-Cat1/α-Cat1 follicle cell clones in the egg chamber display a loss of integrity of the follicle cell epithelium, with loss of cell contacts, adherens junctions and a collapse of the membrane-associated spectrin cytoskeleton. Expression of α-CatΔP.Scer\UAS.P\T.T:Ivir\HA1 or α-CatST-D.Scer\UAS.P\T.T:Ivir\HA1 (but not α-CatST-A.Scer\UAS.P\T.T:Ivir\HA1, α-CatT645D.Scer\UAS.P\T.T:Ivir\HA1 or α-CatT645A.Scer\UAS.P\T.T:Ivir\HA1) under the control of Scer\GAL4twi.PU in a α-Cat1/α-Cat1 mutant background results in a significant reduction in rate of border cell migration in the egg chamber, but migration is still completed during stage 10 of oogenesis, as compared to controls.
α-Cat1 mutant follicular epithelium cells lose cell-cell contacts and adopt a flattened, rounded shape, with loss of adherens junction markers. Border cells fail to migrate and remain at the anterior tip of the follicle.
The cuticle of α-Cat1 mutant embryos shows severe defects in the head skeleton, which result from a failure in head involution. The failure of head involution leaves a large anterior opening in the epidermis through which internal organs are expelled, and the epidermis/cuticle shrinks as a result of muscle contractions. About 68% of embryos display strong head defects and 32% show weak head defects. The ventral and dorsal cuticle appear normal and no obvious defects are observed in other embryonic tissues. Ultrastructural morphology of epithelial adherens junctions is normal in stage 16 α-Cat1. α-Cat1 mutant clones (generated through recombination of the α-CatUbi.PS rescue construct) do not develop in third instar larval imaginal discs. α-Cat1 mutant clones in the ovary (generated through recombination of the α-CatUbi.PS rescue construct) display a variety of oogenesis defects. These include a compromised follicular epithelium leading to fused follicles and failure of border cell migration. The majority of α-Cat1 mutant cells have lost contact with wild type follicle cells. These cells rarely form multilayered clusters, but are usually flattened and tightly attached to the basement membrane. α-Cat1 mutant follicle cells appear to maintain apical-basal polarity, however neighbouring cells appear to lose polarity as their microvillus tufts point in different directions.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
The severity of the embryonic cuticle defects characteristic for α-Cat1 mutants is further enhanced by combination with Df(1)Vinc2 or by expression of sqhDD.Scer\UAS under the control of Scer\GAL4c381 in the mutant background.
Expression of shgScer\UAS.P\T.cSa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU does not suppress the phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are also not rescued. Ubiquitous expression of shgScer\UAS.P\T.cSa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fails to rescue the embryonic head defects and lethality seen in α-Cat1 mutant embryos. Expression of α-Cat::shgScer\UAS.P\T.cSa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are also rescued. Ubiquitous expression of α-Cat::shgScer\UAS.P\T.cSa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic head defects seen in α-Cat1 mutant embryos. Animals are unable to develop beyond the larval stages. Expression of α-Cat::shgΔVH1.Scer\UAS.P\T under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are also rescued. Ubiquitous expression of α-Cat::shgΔVH1.Scer\UAS.P\T under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic head defects seen in α-Cat1 mutant embryos. Animals are unable to develop beyond the larval stages. Expression of α-Cat::shgΔVH3-CTD.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are also partially rescued. Ubiquitous expression of α-Cat::shgΔVH3-CTD.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic head defects seen in α-Cat1 mutant embryos. Animals are unable to develop beyond the larval stages. Expression of α-Cat::bazbazOD.ΔVH1.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU does not suppress the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are also not rescued. Ubiquitous expression of α-Cat::bazbazOD.ΔVH1.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially rescues embryonic head defects seen in α-Cat1 mutant embryos. The lethality is not rescued. Expression of α-Cat::bazΔVH1.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are not rescued. Ubiquitous expression of α-Cat::bazΔVH1.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU does not rescue the head involution defects and lethality seen in α-Cat1 mutant embryos. Expression of α-Cat::bazαCatNbazODC.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are not rescued. Ubiquitous expression of α-Cat::bazαCatNbazODC.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially rescues the embryonic head defects seen in α-Cat1 mutant embryos. The lethality is not rescued. Expression of α-Cat::bazScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are also partially rescued. Ubiquitous expression of α-Cat::bazScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU does not rescue the embryonic head defects and lethality seen in α-Cat1 mutant embryos. Expression of α-Cat::edΔVH1.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU does not suppress the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are not rescued. Ubiquitous expression of α-Cat::edΔVH1.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU does not rescue the embryonic head defects and lethality seen in α-Cat1 mutant embryos.
One copy of shgg119 causes a significant enhancement of the cuticle defects seen in α-Cat1 mutant embryos. >99% of embryos display a strong head defect and 21% show defects in the ventral cuticle. One copy of Arpc1Q25st partially suppresses the cuticle defects seen in α-Cat1 mutant embryos. The phenotype is more severe when Arpc1Q25st is introduced maternally rather than paternally. One copy of Arp3EP3640 partially suppresses the cuticle defects seen in α-Cat1 mutant embryos. Embryos show less severe defects on average and a fraction of embryos have normal heads. One copy of SCARΔ37 partially suppresses the cuticle defects seen in α-Cat1 mutant embryos. Embryos show less severe defects on average and a fraction of embryos have normal heads. The phenotype is more severe when SCARΔ37 is introduced maternally rather than paternally. Expression of shgScer\UAS.P\T.cSa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fails to rescue the embryonic lethality seen in α-Cat1 mutants. Expressing α-Cat::shgScer\UAS.P\T.cSa allows α-Cat1 mutant clones (generated through recombination of the α-CatUbi.PS rescue construct) to develop in third instar larval imaginal discs. The follicle epithelium and border cell migration defects are also rescued. Expressing shgScer\UAS.P\T.cSa does not allow α-Cat1 mutant clones (generated through recombination of the α-CatUbi.PS rescue construct) to develop in third instar larval imaginal discs. Mutant clone cells generated in the follicle epithelium lose cell contacts and border cells fail to migrate. Expression of α-Cat::shgScer\UAS.P\T.cSa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues head morphogenesis and embryonic lethality in 89% of α-Cat1 mutants, but animals die during the larval stages.
Xenogenetic Interactions
Statement
Reference
Expression of Mmus\Ctnna2Scer\UAS.P\T.cDa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are partially rescued. Ubiquitous expression of Mmus\Ctnna2Scer\UAS.P\T.cDa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the head involution defects seen in α-Cat1 mutant embryos. Animals are unable to develop beyond the pupal stages. Expression of Mmus\Ctnna1Scer\UAS.P\T.cDa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are not rescued. Ubiquitous expression of Mmus\Ctnna1Scer\UAS.P\T.cDa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fails to rescue the head involution defects seen in α-Cat1 mutant embryos. Animals are unable to live beyond the embryonic stages. Expression of Mmus\Ctnna2Δ56.Scer\UAS.P\T under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fails to suppress the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are not rescued. Ubiquitous expression of Mmus\Ctnna2Δ56.Scer\UAS.P\T under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially rescues the head involution defects seen in α-Cat1 mutant embryos. Some animals are able to develop to the larval stages.
Complementation and Rescue Data
Comments
Expression of α-CatScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4unspecified rescues the embryonic head morphogenesis and lethality of α-Cat1/α-Cat1 mutants, and rescues the follicular epithelium integrity defects of α-Cat1/α-Cat1 follicle cell clones. Expression of α-CatΔP.Scer\UAS.P\T.T:Ivir\HA1, α-CatT645A.Scer\UAS.P\T.T:Ivir\HA1, α-CatT645D.Scer\UAS.P\T.T:Ivir\HA1, α-CatST-A.Scer\UAS.P\T.T:Ivir\HA1, or α-CatST-D.Scer\UAS.P\T.T:Ivir\HA1, under the control of Scer\GAL4twi.PU rescues the embryonic head morphogenesis and partially rescues the lethality of α-Cat1/α-Cat1 mutants, and partially rescues the follicular epithelium integrity defects of α-Cat1/α-Cat1 follicle cell clones.
Expression of α-CatScer\UAS.P\T.cDa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are also rescued. Ubiquitous expression of α-CatScer\UAS.P\T.cDa under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic head defects and lethality seen in α-Cat1 mutant embryos. Expression of α-CatScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are also rescued. Ubiquitous expression of α-CatScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic head defects and lethality seen in α-Cat1 mutant embryos. Expression of α-CatScer\UAS.P\T.ΔVH1.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fails to rescue the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). Border cell migration does not take place. Ubiquitous expression of α-CatScer\UAS.P\T.ΔVH1.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fails to rescue the embryonic head defects and lethality seen in α-Cat1 mutant embryos. Expression of α-CatScer\UAS.P\T.ΔVH3-CTD.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fails to rescue the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). Border cell migration does not take place. Ubiquitous expression of α-CatScer\UAS.P\T.ΔVH3-CTD.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic head defects seen in α-Cat1 mutant embryos. The lethality is partially rescued. Expression of α-CatScer\UAS.P\T.ΔVH3.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fails to rescue the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). Border cell migration does not take place. Ubiquitous expression of α-CatScer\UAS.P\T.ΔVH3.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fails to rescue the embryonic head defects and lethality seen in α-Cat1 mutant embryos. Expression of α-CatScer\UAS.P\T.ΔCTD.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fully rescues the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). In most cases the border cell migration defects are also rescued. Ubiquitous expression of α-CatScer\UAS.P\T.ΔCTD.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic head defects seen in α-Cat1 mutant embryos. The lethality is partially rescued. Expression of α-CatScer\UAS.P\T.ΔVH2.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially rescues the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are partially rescued. Ubiquitous expression of α-CatScer\UAS.P\T.ΔVH2.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially rescues the head involution defects that result in lethality in α-Cat1 mutant embryos, but the animals are unable to develop beyond the larval stages. Expression of α-CatScer\UAS.P\T.ΔVH2N.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially rescues the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are partially rescued. Ubiquitous expression of α-CatScer\UAS.P\T.ΔVH2N.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic head defects seen in α-Cat1 mutant embryos. No adults are seen but the animals are able to develop beyond the embryonic stages, with some animals reaching the pupal stage. Expression of α-CatScer\UAS.P\T.ΔVH2C.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU fully rescues the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are almost completely rescued. Ubiquitous expression of α-CatScer\UAS.P\T.ΔVH2C.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic head defects seen in α-Cat1 mutant embryos. The lethality is partially rescued; some adults are recovered but many animals are unable to develop beyond the larval and pupal stages. Expression of α-CatScer\UAS.P\T.ΔVIN.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU almost completely rescues the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are also rescued. Ubiquitous expression of α-CatScer\UAS.P\T.ΔVIN.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic head defects seen in α-Cat1 mutant embryos. The lethality is partially rescued; adults are recovered but some animals are unable to develop beyond the larval and pupal stages. Expression of α-CatΔ64.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU partially rescues the follicular epithelium cell phenotypes seen in α-Cat1 mutant follicle cell clones (generated through recombination of the α-CatUbi.PS rescue construct). The border cell migration defects are partially rescued. Ubiquitous expression of α-CatΔ64.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the head involution defects seen in α-Cat1 mutant embryos. The lethality is not rescued; the animals are unable to develop beyond the larval stages.
Expression of α-CatΔCTD.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the lethality seen in α-Cat1 mutant embryos. Expression of α-CatScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL4Act.PU rescues the embryonic lethality seen in α-Cat1 mutants to fertile adults. Expressing α-CatScer\UAS.P\T.T:Ivir\HA1 allows α-Cat1 mutant clones (generated through recombination of the α-CatUbi.PS rescue construct) to develop in third instar larval imaginal discs. The follicle epithelium and border cell migration defects are also rescued.
Images (0)
Mutant
Wild-type
Stocks (20)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)