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General Information
Symbol
Zzzz\GGGGCC30.UAS.EGFP
Species
a. artificial
Name
FlyBase ID
FBal0294759
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-(G4C2)30
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulates expression of 30 GGGGCC repeats (i.e. an expanded number of repeats than normally found in the first intron of the human 'C9orf72' gene, and associated with ALS and FTD diseases) following by the coding sequence for EGFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
is exacerbated by eRF1GD4704
is ameliorated by tauKO
is exacerbated by MhcHMS01471
is exacerbated by MhcJF02069
is exacerbated by MhcKK102402
is ameliorated by RanGAPSd
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

FlyBase curator comment: "FTDALS1" is associated with G4C2 transcripts and dipeptide repeat proteins derived from human C9ORF72.

Disease-implicated variant(s)
 
This allele represents a human variant implicated in disease.

This construct encodes only the RNA repeat motif.

C9orf72:n.intron1[30GGGGCC]
Variants Synonym(s)
C9ORF72, (GGGGCC)n REPEAT EXPANSION
C9ORF72, (G4C2)n REPEAT EXPANSION
External database links
Comments concerning this variant
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Zzzz\GGGGCC30.UAS.EGFP under the control of Scer\GAL4GMR.long induces eye necrosis.

Expression under the control of Scer\GAL4VGlut-OK371 results in decreased adult climbing activity.

Expressing Zzzz\GGGGCC30.UAS.EGFP under the control of Scer\GAL4GMR.PU leads to eye defect: weak roughness and slight disruptions to the ommatidial array, with associated loss of pigmentation.

Expression of Zzzz\GGGGCC30.UAS.EGFP under the control of Scer\GAL4GMR.PU leads to degenerative eyes with necrotic spots and climbing response defects.

Expression of Zzzz\GGGGCC30.UAS.EGFP under the control of Scer\GAL4elav-C155 leads to delayed firing discharge in flight dorsal muscles after stimulation (200 Hz for 2 s) of giant fiber neurons into the brain.

Expression of Zzzz\GGGGCC30.UAS.EGFP under the control of Scer\GAL4GMR.PU leads to degeneration of eyes.

Expression of Zzzz\GGGGCC30.UAS.EGFP under the control of Scer\GAL4GMR61G12 leads to a progressive loss of axon bundles from sLNv neurons and climbing defects in adults. Its expression under the control of Scer\GAL4elav.PLu leads to a reduction in the number of active zones of muscle 6/7 in abdominal segments A2 and A3 of NMJs of third instar larvae.

The expression of Hsap\FUSR521C.UAS.Tag:HA under the control of Scer\GAL4GMR.PF leads to a rough eye phenotype, with depigmentation.

Scer\GAL4GMR.long-mediated expression of Zzzz\GGGGCC30.Scer\UAS.T:Avic\GFP-EGFP severely disrupts eye morphology: cell death, loss of pigmentation and ommatidial disruption are observed. Aged flies show increased disruption.

At 28 days, but not 7 days, after eclosion, Scer\GAL4VGlut-OK371 Zzzz\GGGGCC30.Scer\UAS.T:Avic\GFP-EGFP flies show a significant reduction in locomotor activity compared to controls.

14 day old eyes of Scer\GAL4GMR.long Zzzz\GGGGCC30.Scer\UAS.T:Avic\GFP-EGFP flies contain Pur-α-positive inclusions.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by Mtp[+]/Mtpd07488

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by CenG1Ac06703/CenG1A[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by Epac[+]/Epace00785

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by CG4393[+]/CG4393c04473

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by CG32069e00355/CG32069[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by Prp18c07155/Prp18[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by Imp[+]/Impd08578

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by DCTN5-p25[+]/DCTN5-p25f02738

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by sNPFc07042/sNPF[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by GCC185[+]/GCC185e02741

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by Kpc1c06744/Kpc1[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by NetA[+]/NetAd04075

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by Ptpmeg[+]/Ptpmegc06293

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by kugc05977/kug[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by c04797c04797/c04797[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by srac03600/sra[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by CG8004d09179/CG8004[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-enhanceable by GstS1e00777/GstS1[+]

Suppressed by
Statement
Reference

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, suppressible | partially by SNF4Aγd05297/SNF4Agamma[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, suppressible by Hsc70Cb[+]/Hsc70Cbd03562

NOT suppressed by
Statement
Reference

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by srac03600/sra[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by CG8004d09179/CG8004[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by GstS1e00777/GstS1[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by Mtp[+]/Mtpd07488

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by CenG1Ac06703/CenG1A[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by Epac[+]/Epace00785

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by CG4393[+]/CG4393c04473

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by CG32069e00355/CG32069[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by Prp18c07155/Prp18[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by Imp[+]/Impd08578

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by DCTN5-p25[+]/DCTN5-p25f02738

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by sNPFc07042/sNPF[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by GCC185[+]/GCC185e02741

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by Kpc1c06744/Kpc1[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by NetA[+]/NetAd04075

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by Ptpmeg[+]/Ptpmegc06293

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by kugc05977/kug[+]

Scer\GAL4GMR.PU, Zzzz\GGGGCC30.UAS.EGFP has eye phenotype, non-suppressible by c04797c04797/c04797[+]

Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Zzzz\GGGGCC30.Scer\UAS.T:Avic\GFP-EGFP
Zzzz\GGGGCC30.UAS.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (15)