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General Information
Symbol
Hsap\MECP2UAS.FL
Species
H. sapiens
Name
FlyBase ID
FBal0294765
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-MeCP2, UAS-hMeCP2
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulates expression of a wild type, full length cDNA of the Hsap\MECP2 e2 isoform (1461 nt, 486 aa).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Hsap\MECP2Scer\UAS.FL driven by Scer\GAL4elav-C155 (or Scer\GAL4futsch-C380, along with Scer\GAL80ChAT.3.3P) causes a significant decrease in dendritic branching (reduces dendritic length and dendritic branch number) in MN5 motoneurons.

Expression of Hsap\MECP2R294X.Scer\UAS under the control of Scer\GAL4futsch-C380 has no effect on morphology or survival of the motoneurons 5 in the pupal ventral nerve cord.

Overexpression of Hsap\MECP2Scer\UAS.FL driven by Scer\GAL4GMR.PU leads to a rough eye phenotype, with ommatidial disorganization.

Expression of Hsap\MECP2Scer\UAS.FL in MN5 motoneurons (via Scer\GAL4futsch-C380 and Scer\GAL80Cha.3.3P) significantly impairs new dendrite formation, resulting in a reduction of total dendritic length by 50%. However, membrane excitability, dendritic territory borders, and dendritic branch elongation are unaffected.

Flies expressing Hsap\MECP2Scer\UAS.FL in MN5 motoneurons (via Scer\GAL4futsch-C380 and Scer\GAL80Cha.3.3P) show a significant reduction in mean duration per flight bout and total flight duration compared to controls. In contrast, no difference is seen between groups for MN5 firing patterns or wing beat frequency during flight, or the likelihood to engage in a flight.

Expression via Scer\GAL4GMR.PU disrupts the external structure of the eye, resulting in a 'glassy' effect on the surface. Ommatidia are disorganized and interommatidial bristles are partially lost.

Expression via Scer\GAL4C5 produces extra vein tissue around veins L3 and L5.

Expression via Scer\GAL4Cha.7.4 leads to impaired motor function in adult flies, as measured in a climbing assay.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible | partially by Ube3a[+]/Ube3a6J

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has ommatidium phenotype, suppressible | partially by Ube3a[+]/Ube3a6J

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible | partially by Ube3a[+]/Ube3a15B

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible | partially by Ube3a8O/Ube3a[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has ommatidium phenotype, suppressible | partially by Ube3a8O/Ube3a[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by crolc04670/crol[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by crol[+]/crole04017

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by osa00090/osa[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by osa[+]/osa308

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by osa[+]/osaEY09619

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by pbl[+]/pbl2

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by pbl[+]/pbl3

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by pbl[+]/pbl5

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by pbl[+]/pbl09645

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by Scme01989/Scm[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by ScmD1/Scm[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by ScmET50/Scm[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by crold03416/crol[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by Scm[+]/ScmM36

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by ScmM56/Scm[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by Scm4/Scm[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by Smre04377/Smr[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by Smre04389/Smr[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by Asx[+]/AsxEY07384

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by AsxXF23/Asx[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by corto07128b/corto[+]

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by corto[+]/cortoc03244

Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU has eye phenotype, suppressible by cortoe02822/corto[+]

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of kibraGD7772 significantly suppresses the decreases in dendritic branch length (fully) and number (partially) in MN5 motoneurons with expression of Hsap\MECP2Scer\UAS.FL driven by Scer\GAL4futsch-C380 (along with Scer\GAL80ChAT.3.3P).

Ube3a6J/+, Ube3a15B/+ or Ube3a8O/+ suppress rough eye phenotypes with ommatidial disorganization in flies with expression of Hsap\MECP2Scer\UAS.FL driven by Scer\GAL4GMR.PU.

Introduction of osa00090/+ significantly increases total MN5 dendritic length and dendritic number above that in the Hsap\MECP2Scer\UAS.FL, Scer\GAL4futsch-C380, Scer\GAL80Cha.3.3P background.

Complementation and Rescue Data
Comments

The apoptosis of motoneurons MN5 in the pupal nerve cord observed upon expression of Hsap\MECP2R294X.Scer\UAS under the control of Scer\GAL4futsch-C380 is not suppressed by co-expression of Hsap\MECP2Scer\UAS.FL.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\MECP2Scer\UAS.FL
Hsap\MECP2UAS.FL
Name Synonyms
Secondary FlyBase IDs
    References (7)