Insertion of a Mi{Hto-WP} element within Sfp24Bd ( 2L:3 ,669,091 (+) -Release 6). The hostile takeover (hto) exon in the Mi{Hto-WP} element splices to the downstream Drgx exon 2. This results in a fusion protein being expressed under the control of Scer\UAS regulatory sequences in the Mi{Hto-WP} element. The fusion protein consists of 3 copies of the T:Zzzz\FLAG tag, the mCherry form of Disc\RFP, 9 amino acid linker sequence translated from the Drgx 5'UTR exon (LGPAAAGRG) and the Drgx coding sequence. Sfp24Bd and the nearby Sfp24Ba, Sfp24Bb and Sfp24Bc genes are not induced because they are transcribed in the reverse direction.
Expression of one copy of DrgxGLO under the control of Scer\GAL4GMR.PU produces flies that have severely rough eyes.
Expression of DrgxGLO under the control of Scer\GAL4ey.PU results in eye elimination and a reduced head.
Expression of DrgxGLO under the control of Scer\GAL4Bx-MS1096 suppresses wing vein development.
When DrgxGLO-expressing clones are generated in follicle cells at stages 8-10 of oogenesis under the control of Scer\GAL4Act5C.PP) the oocyte follicle cells (OFCs) fail to translocate posteriorly towards the oocyte as they do in wild type. The DrgxGLO-expressing cells over the nurse cells flatten correctly, but do not spread as they do in wild type. In posterior OFC clones, the basal circumferential actin cables become highly disorganized and the clones lack the hexagonal pattern in wild type, instead showing smooth clone borders, indicating a defect in adhesion. The apical-basal organisation of the clones is also disrupted. No cell death is observed.
Generated using the hostile takeover (Hto) system.