Deletion of the C-tail region.
Scer\GAL4btl.PU/shotRE-ΔCtail.UAS.GFP fails to rescue shot3
shotRE-ΔCtail.UAS.GFP/Scer\GAL4sca-537.4 fails to rescue Df(2R)MK1/shotSF20
shotRE-ΔCtail.UAS.GFP/Scer\GAL4eve.RN2O fails to rescue shotunspecified
Scer\GAL4sr-md710/shotRE-ΔCtail.UAS.GFP fails to rescue shotunspecified
The expression of shotRE-ΔCtail.Scer\UAS.T:Avic\GFP rescues both the increased length:width ratio of the dorsal hole (expression under the control of Scer\GAL4pnr.PU) and the abnormal microtubules of the dorsal-most epithelial cells (expression under the control of Scer\GAL4en.PU) observed during dorsal closure of shotSF20 homozygous embryos.
Expression of shotRE-ΔCtail.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4eve.RN2O does not rescue the motor neuron stall phenotype seen in shotunspecified embryos in vivo and expression under the control of Scer\GAL4sca-537.4 fails to rescue the axon extension and microtubule organisation defects of shotSF20/Df(2R)MK1 embryonic neurons in primary culture.
Expression of shotRE-ΔCtail.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4sr-md710 fails to rescue tendon cell integrity in shotunspecified embryos.