FB2025_01 , released February 20, 2025
Allele: Dmel\shotRE-3MtLSmut.UAS.GFP
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General Information
Symbol
Dmel\shotRE-3MtLSmut.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0295415
Feature type
allele
Associated gene
Dmel\shot
Associated Insertion(s)
Carried in Construct
PBac{UAS-shot.RE-3MtLSmut-GFP}
Key Links
Transgenic product class
missense_variant
(Alves-Silva et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Alves-Silva et al., 2012)
Mutagen
in vitro construct
(Alves-Silva et al., 2012)
Progenitor genotype
shotLA.UAS.GFP
(Alves-Silva et al., 2012)
Carried in construct
PBac{UAS-shot.RE-3MtLSmut-GFP}
Cytology
Description

Mutation of the three putative microtubule tip localisation sequence (MtLS) motifs in the Ctail region. In each case the putative MtLS motif is replaced by four Ala residues. The location of each MtLS motif is as follows: amino acid residues 4966-4969, 5077-5080 and 5141-5144 (coordinates numbered according to FBpp0086744).

(Alves-Silva et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
shot
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4en.PU/shotRE-3MtLSmut.UAS.GFP rescues shotSF20

      (Takács et al., 2017)

      Scer\GAL4sr-md710/shotRE-3MtLSmut.UAS.GFP rescues shotunspecified

      (Alves-Silva et al., 2012)
      Fails to rescue

      Scer\GAL4sca-537.4/shotRE-3MtLSmut.UAS.GFP fails to rescue Df(2R)MK1/shotSF20

      (Alves-Silva et al., 2012)

      shotRE-3MtLSmut.UAS.GFP/Scer\GAL4eve.RN2O fails to rescue shotunspecified

      (Alves-Silva et al., 2012)
      Comments

      The expression of shotRE-3MtLSmut.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4en.PU rescues the abnormal microtubules observed in the dorsal-most epithelial cells of shotSF20 homozygous embryos during dorsal closure.

      (Takács et al., 2017)

      Expression of shotRE-3MtLSmut.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4eve.RN2O does not rescue the motor neuron stall phenotype seen in shotunspecified embryos in vivo and expression under the control of Scer\GAL4sca-537.4 fails to rescue the axon extension and microtubule organisation defects of shotSF20/Df(2R)MK1 embryonic neurons in primary culture.

      Expression of shotRE-3MtLSmut.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4sr-md710 restores tendon cell integrity in shotunspecified embryos.

      (Alves-Silva et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      shotRE-3MtLSmut.Scer\UAS.T:Avic\GFP
      shotRE-3MtLSmut.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)