4.5kb of genomic sequence from upstream of the wake translation start site is fused upstream of a GAL4 driver.
Scer\GAL4wake.4.5 drives expression in all Pdf-expressing LNv- and in a subset of LNd- and DN-neurons. KAEDE experiments suggest that Scer\GAL4wake.4.5 expression cycles throughout the day with a higher expression during the night.
sleep defective, with Scer\GAL80Pdf.PS, wakemiRNA.UAS.1
sleep defective, with wakemiRNA.UAS.1
sleep defective, with wakemiRNA.UAS.2