FB2025_01 , released February 20, 2025
Allele: Dmel\IdhR195H.UAS.Tag:FLAG
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General Information
Symbol
Dmel\IdhR195H.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0295875
Feature type
allele
Associated gene
Dmel\Idh
Associated Insertion(s)
Carried in Construct
P{UAS-Idh.R195H.FLAG}
Key Links
Genomic Maps

Transgenic product class
missense_variant
(Reitman, 2014.4.30)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Reitman, 2014.4.30)
Mutagen
in vitro construct
(Reitman, 2014.4.30)
Progenitor genotype
Carried in construct
P{UAS-Idh.R195H.FLAG}
Cytology
Description

Amino acid replacement: R195H.

(Reitman, 2014.4.30)

UAS regulatory sequences drive expression of mutated Idh sequences tagged with Tag:FLAG.

(Reitman, 2014.4.30)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Idh
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:8,357,511..8,357,511 [-]
Nucleotide change:

G8357511A

Amino acid change:

R132H | Idh-PA; R166H | Idh-PB; R185H | Idh-PC; R153H | Idh-PD; R132H | Idh-PF; R166H | Idh-PG; R166H | Idh-PI; R166H | Idh-PJ; R195H | Idh-PK

Reported amino acid change:

R195H

Comment:

Analogous mutation in human IDH2 implicated in cancer, multiple, IDH1,2-related; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.

(Reitman et al., 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  D-2-hydroxyglutaric aciduria
      CEA
      (Reitman et al., 2015)
      model of  cancer
      CEA
      (Reitman et al., 2015)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
       

      Overexpression of IdhR195H.Scer\UAS.T:Zzzz\FLAG leads to the overproduction of D-2-hydroxyglutarate (D-2HG).

      (Reitman et al., 2015)
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Reitman et al., 2015)
      IDH1:p.Arg132His
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: IDH1_156444
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Pan-neuronal expression of IdhR195H.Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav-C155 leads to defects in wing-expansion, cuticle-tanning, and locomotion.

      When IdhR195H.Scer\UAS.T:Zzzz\FLAG is expressed in CCAP neurons using Scer\GAL4CCAP.PP, flies display defects in wing-expansion and cuticle-tanning with 50% penetrance.

      The number of mature crystal cells is increased in larvae expressing Scer\GAL4Hml.Δ>IdhR195H.Scer\UAS.T:Zzzz\FLAG compared with wild-type. The number of lamellocytes is also increased due to Scer\GAL4Hml.Δ>IdhR195H.Scer\UAS.T:Zzzz\FLAG.

      Expression of IdhR195H.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4dome-PG14 results in smaller lymph glands in third instar larvae compared with wild-type and the near-absence of differentiated cortical hemocytes.

      (Reitman et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4elav-C155 has adult cuticle phenotype, suppressible by D2hgdhUAS.Tag:FLAG, Scer\GAL4elav-C155

      (Reitman et al., 2015)

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4elav-C155 has wing phenotype, suppressible by D2hgdhUAS.Tag:FLAG, Scer\GAL4elav-C155

      (Reitman et al., 2015)

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4CCAP.PP has wing phenotype, suppressible by IdhRNAi.UAS.cUa, Scer\GAL4CCAP.PP

      (Reitman et al., 2015)

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4CCAP.PP has adult cuticle phenotype, suppressible by D2hgdhUAS.Tag:FLAG, Scer\GAL4CCAP.PP

      (Reitman et al., 2015)

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4CCAP.PP has wing phenotype, suppressible by D2hgdhUAS.Tag:FLAG, Scer\GAL4CCAP.PP

      (Reitman et al., 2015)

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4CCAP.PP has wing phenotype, suppressible by Sod2UAS.cMa, Scer\GAL4CCAP.PP

      (Reitman et al., 2015)

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4CCAP.PP has wing phenotype, suppressible by CatUAS.cAa, Scer\GAL4CCAP.PP

      (Reitman et al., 2015)

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4CCAP.PP has wing phenotype, suppressible | partially by DroncRNAi.UAS.cUa, Scer\GAL4CCAP.PP

      (Reitman et al., 2015)

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4CCAP.PP has wing phenotype, suppressible | partially by DarkRNAi.UAS.cUa, Scer\GAL4CCAP.PP

      (Reitman et al., 2015)

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4CCAP.PP has wing phenotype, suppressible | partially by Diap1UAS.cUa, Scer\GAL4CCAP.PP

      (Reitman et al., 2015)

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4CCAP.PP has wing phenotype, suppressible by p53RNAi.UAS.cUa, Scer\GAL4CCAP.PP

      (Reitman et al., 2015)
      Enhancer of
      Statement
      Reference

      IdhR195H.UAS.Tag:FLAG, Scer\GAL4GMR.PFa is an enhancer of eye phenotype of Scer\GAL4GMR.PFa, rprUAS.cUa

      (Reitman et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      IdhR195H.Scer\UAS.T:Zzzz\FLAG
      IdhR195H.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (4)