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General Information
Symbol
Dmel\IdhR195H.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0295875
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
G8357511A
Amino acid change:
R153H | Idh-PD; R185H | Idh-PC; R166H | Idh-PG; R166H | Idh-PB; R166H | Idh-PI; R166H | Idh-PJ; R132H | Idh-PF; R132H | Idh-PA; R195H | Idh-PK
Reported amino acid change:
R195H
Comment:
Analogous mutation in human IDH2 implicated in cancer, multiple, IDH1,2-related; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: R195H.
UAS regulatory sequences drive expression of mutated Idh sequences tagged with Tag:FLAG.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 
Overexpression of IdhR195H.Scer\UAS.T:Zzzz\FLAG leads to the overproduction of D-2-hydroxyglutarate (D-2HG).
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Pan-neuronal expression of IdhR195H.Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav-C155 leads to defects in wing-expansion, cuticle-tanning, and locomotion. When IdhR195H.Scer\UAS.T:Zzzz\FLAG is expressed in CCAP neurons using Scer\GAL4CCAP.PP, flies display defects in wing-expansion and cuticle-tanning with 50% penetrance. The number of mature crystal cells is increased in larvae expressing Scer\GAL4Hml.Δ>IdhR195H.Scer\UAS.T:Zzzz\FLAG compared with wild-type. The number of lamellocytes is also increased due to Scer\GAL4Hml.Δ>IdhR195H.Scer\UAS.T:Zzzz\FLAG. Expression of IdhR195H.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4dome-PG14 results in smaller lymph glands in third instar larvae compared with wild-type and the near-absence of differentiated cortical hemocytes.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
IdhR195H.Scer\UAS.T:Zzzz\FLAG
IdhR195H.UAS.Tag:FLAG
Name Synonyms
Secondary FlyBase IDs
    References (2)