FB2025_01 , released February 20, 2025
Allele: Dmel\fogUAS.cRa
Open Close
General Information
Symbol
Dmel\fogUAS.cRa
Species
D. melanogaster
Name
FlyBase ID
FBal0296413
Feature type
allele
Associated gene
Dmel\fog
Associated Insertion(s)
Carried in Construct
P{UAS-fog.WT}
Also Known As
UAS-fog
Key Links
Transgenic product class
cDNA
(Ratnaparkhi and Zinn, 2007)
wild_type
(Ratnaparkhi and Zinn, 2007)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Ratnaparkhi and Zinn, 2007)
wild_type
(Ratnaparkhi and Zinn, 2007)
Mutagen
in vitro construct
(Ratnaparkhi and Zinn, 2007)
Progenitor genotype
Carried in construct
P{UAS-fog.WT}
Cytology
Description

UASt regulatory sequences drive expression of 1.6kb of fog cDNA sequences (full-length).

(Ratnaparkhi and Zinn, 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
fog
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of fogScer\UAS.cRa under the control of Scer\GAL4Bx-MS1096 results in a strong wing defect in male flies, with complete penetrance. The wings appear short, sometimes stub-like, and at times necrotic. By contrast, female flies appear normal. However, increasing the dosage of fog by either increasing the copy number of Scer\GAL4Bx-MS1096 or fogScer\UAS.cRa leads to wing defects in these females as well.

      Animals expressing fogScer\UAS.cRa under the control of Scer\GAL4Mhc.PU exhibit small mitochondria that are spherical or elliptical in shape, indicating fragmentation. This effect is observed in animals raised at both 25[o]C and 28[o]C.

      (Ratnaparkhi, 2013)

      Overexpression of fogScer\UAS.cRa in the developing glia, under the control of Scer\GAL4repo results in 1st or 2nd instar lethality. Mutant embryos exhibit severe glial defects. The longitudinal glia exhibit a disorganised and clumped appearance, and the extension of glial processes appears abnormal.

      Overexpression of fogScer\UAS.cRa in neurons under the control of Scer\GAL4elav.PLu or Scer\GAL4elav-C155 generates strong central nervous system defects. The three pairs of 1D4-positive longitudinal axon bundles in the CNS display multiple breaks and fusions and fail to extend along straight pathways. Ectopic midline crossing by 1D4-positive longitudinal axon bundles is observed in 27% of segments. In embryos carrying both Scer\GAL4elav.PLu and Scer\GAL4elav-C155, the percentage of segments with ectopic midline crossing increases to 44%. SNa defects are also observed.

      Overexpression of fogScer\UAS.cRa in glia, under the control of Scer\GAL4repo, causes axon guidance defects, producing ectopic midline crossing phenotypes in 23% of segments.

      (Ratnaparkhi and Zinn, 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing phenotype, suppressible | partially by rho-7pΔ1

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing | male limited phenotype, suppressible | partially by rho-7pΔ1

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing phenotype, suppressible | partially by Drp1GD10456, Scer\GAL4Bx-MS1096

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing | male limited phenotype, suppressible | partially by Drp1GD10456, Scer\GAL4Bx-MS1096

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing | male limited phenotype, suppressible by MarfUAS.cDa, Scer\GAL4Bx-MS1096

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing phenotype, suppressible by MarfUAS.cDa, Scer\GAL4Bx-MS1096

      (Ratnaparkhi, 2013)

      Scer\GAL4Mhc.PU, fogUAS.cRa has mitochondrion phenotype, suppressible by Drp1GD10456, Scer\GAL4Mhc.PU

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing phenotype, suppressible by WASpUAS.cUa/Scer\GAL4Bx-MS1096

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing | male limited phenotype, suppressible by WASpUAS.cUa/Scer\GAL4Bx-MS1096

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing phenotype, suppressible | partially by rho-7GD3775/Scer\GAL4Bx-MS1096

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing | male limited phenotype, suppressible | partially by rho-7GD3775/Scer\GAL4Bx-MS1096

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing phenotype, suppressible by cta5/cta[+]

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing | male limited phenotype, suppressible by cta5/cta[+]

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing phenotype, suppressible by Ptp52F[+]/Ptp52F18.3

      (Ratnaparkhi, 2013)

      Scer\GAL4Bx-MS1096, fogUAS.cRa has wing | male limited phenotype, suppressible by Ptp52F[+]/Ptp52F18.3

      (Ratnaparkhi, 2013)

      Scer\GAL4elav-C155, Scer\GAL4elav.PLu, fogUAS.cRa has larval central nervous system phenotype, suppressible by Ptp52F18.3

      (Ratnaparkhi and Zinn, 2007)

      Scer\GAL4elav-C155, Scer\GAL4elav.PLu, fogUAS.cRa has larval segmental nerve branch SNa of A1-7 phenotype, suppressible by Ptp52F18.3

      (Ratnaparkhi and Zinn, 2007)

      Scer\GAL4elav-C155, Scer\GAL4elav.PLu, fogUAS.cRa has fascicle phenotype, suppressible by Ptp52F18.3

      (Ratnaparkhi and Zinn, 2007)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of rho-7GD3775 results in a 66-74% suppression of the wing phenotype found upon expression of fogScer\UAS.cRa under the control of Scer\GAL4Bx-MS1096.

      The presence of cta5 strongly suppresses the wing defects seen in male flies expressing fogScer\UAS.cRa under the control of Scer\GAL4Bx-MS1096.

      The presence of Ptp52F18.3 strongly suppresses the wing defects seen in male flies expressing fogScer\UAS.cRa under the control of Scer\GAL4Bx-MS1096.

      Approximately 43.5% of Scer\GAL4Bx-MS1096>fogScer\UAS.cRa/rho-7pΔ1 males show rescue of the wing phenotype whereas 37% show only a partial rescue, indicating that rho-7 is a suppressor of fog signalling.

      Expression of Drp1GD10456 results in a 77-83% suppression of the wing phenotype found in male flies expressing fogScer\UAS.cRa under the control of Scer\GAL4Bx-MS1096, suggesting that loss of mitochondrial fission inhibits fog signalling.

      Overexpression of Drp1Scer\UAS.cDa under the control of Scer\GAL4Bx-MS1096, enhances the wing phenotype of Scer\GAL4Bx-MS1096>fogScer\UAS.cRa flies, with males appearing similar to Scer\GAL4Bx-MS1096>fogScer\UAS.cRa males, with corresponding females showing strong wing defects ranging from relatively mild margin defects to strong wing defects that are similar to those in males.

      Expression of MarfScer\UAS.cDa under the control of Scer\GAL4Bx-MS1096 suppresses the wing phenotype found upon expression of fogScer\UAS.cRa : in >80% of F1 males, the wings appear normal, whereas the F1 females do not show any wing defects.

      Expression of MarfJF01650 under the control of Scer\GAL4Bx-MS1096, leads to a clear enhancement of the wing phenotype found in flies expressing fogScer\UAS.cRa, with complete penetrance: in males, the wings appear small or stub-like, similar to parental males, and all females show short and abnormal wings, indicating that inhibiting mitochondrial fusion through down-regulation of Marf potentiates fog signalling.

      Expression of Drp1GD10456 suppresses the mitochondrial defects found upon expression of fogScer\UAS.cRa under the control of Scer\GAL4Mhc.PU.

      Expression of WASpScer\UAS.cUa in Scer\GAL4Bx-MS1096->fogScer\UAS.cRa animals suppresses the wing phenotype in approximately 53.3% of animals.

      Co-expression of WASpScer\UAS.cUa and fogScer\UAS.cRa, under the control of Scer\GAL4Mhc.PU results in long filamentous mitochondria as opposed to the fragmented forms associated with fogScer\UAS.cRa.

      (Ratnaparkhi, 2013)

      Removal of Ptp52F function in fogScer\UAS.cRa overexpression mutants, through a Ptp52F18.3 mutant background, results in a strong suppression of the CNS phenotype. Only 3% of segments exhibit ectopic midline crossing, as compared to 44% for fogScer\UAS.cRa overexpression (under the control of Scer\GAL4elav-C155 and Scer\GAL4elav.PLu) in a wild-type background. For embryos with one copy of the driver (Scer\GAL4elav.PLu), the phenotype was suppressed from 27% to 5%. The longitudinal tracts appear normal in fogScer\UAS.cRa, Ptp52F18.3 embryos, resembling those seen in Ptp52F18.3 single mutants.

      (Ratnaparkhi and Zinn, 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      fogScer\UAS.cRa
      fogUAS.cRa
      Name Synonyms
      Secondary FlyBase IDs
        References (2)