P-element mediated recombination between P{SUPor-P}KG04646 and P{EPgy2}CG8613EY06332 has resulted in deletion of part of the Shroom coding region. The 5' breakpoint is between 10,217,297 and 10,238,252 and the 3' breakpoint is between 10,238,326 and 10,239,796. The allele retains sequences immediately outside of the two P elements as well as sequences in the 5' coding region, likely because of gap repair. Sequences within the last three exons of Shroom, which encode the Rho-kinase-binding domain, are removed.
ShroomΔ11 mutant stage 8 embryos have reduced convergent extension resulting in a smaller increase in tissue length to width ratio compared to wild type. In contrast, tissue length along the anterior posterior axis is only slightly reduced. ShroomΔ11 mutants exhibit defects in convergent extension cell rearrangement: there is a strong reduction in rosette behaviors (resulting from the contraction of multiple consecutive edges), but little change in local neighbour exchange (resulting from single edge contraction events). Cells in ShroomΔ11 mutants have more neighbours on average midway through elongation. Intercalating cells in ShroomΔ11 mutant embryos have a slightly larger apical area during elongation, with no difference between wild type and mutant cells before elongation.