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General Information
Symbol
Dmel\cazVDRC.cUa
Species
D. melanogaster
Name
FlyBase ID
FBal0296654
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of an RNAi construct targeting caz.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of cazVDRC.cUa under the control of Scer\GAL4elav.PLu results in reduced locomotive capacity in both larvae and adults, decreased adult lifespan as well as reduced total branch length and bouton number in larval neuromuscular junctions. Expression under the control of Scer\GAL4GMR.PS results in rough eye phenotype with fused ommatidia in adult flies.

Expression of Scer\GAL4GMR.PS>cazVDRC.cUa in eye imaginal discs induces morphologically aberrant rough eyes. Scanning electron microscope (SEM) images show fusion of ommatidia and loss of mechanosensory bristles.

Neuron-specific expression of cazVDRC.cUa under the control of Scer\GAL4elav.PLu results in a significantly decreased climbing ability compared with wild-type. The locomotive defect is progressive.

The life span of flies expressing Scer\GAL4elav.PLu>cazVDRC.cUa is similar to that of controls.

The total length of synaptic branches of motor neurons is significantly decreased in Scer\GAL4elav.PLu>cazVDRC.cUa larvae compared with controls.

Compared with wild-type, the average number of synaptic boutons per motor neuron is also significantly decreased as a result of Scer\GAL4elav.PLu>cazVDRC.cUa-expression.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The eye morphology defects (rough appearance with fused ommatidia) characteristic for flies expressing cazVDRC.cUa under the control of Scer\GAL4GMR.PS can be rescued by co-expression of HsrωdsRNA.Sym.Scer\UAS and further worsened by co-expression of HsrωEP93D (which on its own also causes eye defects).

Third instar larvae simultaneously expressing both HsrωdsRNA.Sym.Scer\UAS and cazVDRC.cUa under the control of Scer\GAL4elav.PLu display crawling ability deficiency as well as morphological defects in the neuromuscular junctions (reduced total synaptic branch length and number of boutons) but the severity of each of these defects is not significantly different from either of the single knockdowns.

Heterozygous TER94k15502 enhances the rough-eye phenotype induced by Scer\GAL4GMR.PS>cazVDRC.cUa.

Heterozygous TER9403775 enhances the rough-eye phenotype induced by Scer\GAL4GMR.PS>cazVDRC.cUa.

Expression of Scer\GAL4GMR.PS>cazVDRC.cUa in the presence of heterozygous Df(2R)X1 results in lethality at the pupal stage.

Co-expression of TER94Scer\UAS.cHa suppresses the rough-eye phenotype induced by Scer\GAL4GMR.PS>cazVDRC.cUa.

Flies carrying TER94k15502 and Scer\GAL4elav.PLu>cazVDRC.cUa have significantly worse locomotive ability than do flies with Scer\GAL4elav.PLu>cazVDRC.cUa alone.

Flies co-expressing TER94Scer\UAS.cHa have significantly better climbing ability than do flies expressing Scer\GAL4elav.PLu>cazVDRC.cUa alone.

The decreased branch length phenotype caused by Scer\GAL4elav.PLu>cazVDRC.cUa in motor neurons is significantly enhanced by TER94k15502/+.

The decrease in the number of synaptic boutons in Scer\GAL4elav.PLu>cazVDRC.cUa-expressing larvae is significantly enhanced by TER94k15502/+. However, there is significant differences in the size of synaptic boutons among these genotypes.

Total branch length phenotype caused by Scer\GAL4elav.PLu>cazVDRC.cUa in motor neurons is significantly suppressed by the co-expression of TER94Scer\UAS.cHa. The number of synaptic boutons is also significantly increased in Scer\GAL4elav.PLu>cazVDRC.cUa larvae co-expressing TER94Scer\UAS.cHa compared with larvae expressing Scer\GAL4elav.PLu>cazVDRC.cUa alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
cazVDRC.cUa
Name Synonyms
Secondary FlyBase IDs
    References (3)