FlyBase Allele Report: Dmel\caz[VDRC.cUa]

General Information

Symbol

Dmel\caz^VDRC.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0296654

Feature type

allele

Associated gene

Dmel\caz

Associated Insertion(s)

Carried in Construct

P{VDRC.caz.U}

Key Links

Transgenic product class

RNAi_reagent

(Azuma et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Azuma et al., 2014)

Mutagen

in vitro construct

(Azuma et al., 2014)

Progenitor genotype

Carried in construct

P{VDRC.caz.U}

Cytology

Description

UAS regulatory sequences drive expression of an RNAi construct targeting caz.

(Azuma et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

caz

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis

CEA

(Lo Piccolo and Yamaguchi, 2017, Azuma et al., 2014)

model of amyotrophic lateral sclerosis type 6

CEA

(Wakisaka et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis

is exacerbated by TER94^k15502

(Azuma et al., 2014)

is exacerbated by TER94⁰³⁷⁷⁵

(Azuma et al., 2014)

is ameliorated by TER94^UAS.cHa

(Azuma et al., 2014)

model of amyotrophic lateral sclerosis type 6

is exacerbated by aub^UASp.mGFP6

(Wakisaka et al., 2019)

is ameliorated by AGO3^t2

(Wakisaka et al., 2019)

is exacerbated by rhi⁰²⁰⁸⁶

(Wakisaka et al., 2019)

is ameliorated by AGO3^t3

(Wakisaka et al., 2019)

is exacerbated by rhi^KG00910

(Wakisaka et al., 2019)

is ameliorated by aub^HN2

(Wakisaka et al., 2019)

is ameliorated by aub^QC42

(Wakisaka et al., 2019)

is exacerbated by wde^EY00884

(Wakisaka et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of caz^VDRC.cUa under the control of Scer\GAL4^elav.PLu results in reduced locomotive capacity in both larvae and adults, decreased adult lifespan as well as reduced total branch length and bouton number in larval neuromuscular junctions. Expression under the control of Scer\GAL4^GMR.PS results in rough eye phenotype with fused ommatidia in adult flies.

(Lo Piccolo and Yamaguchi, 2017)

Expression of Scer\GAL4^GMR.PS>caz^VDRC.cUa in eye imaginal discs induces morphologically aberrant rough eyes. Scanning electron microscope (SEM) images show fusion of ommatidia and loss of mechanosensory bristles.

Neuron-specific expression of caz^VDRC.cUa under the control of Scer\GAL4^elav.PLu results in a significantly decreased climbing ability compared with wild-type. The locomotive defect is progressive.

The life span of flies expressing Scer\GAL4^elav.PLu>caz^VDRC.cUa is similar to that of controls.

The total length of synaptic branches of motor neurons is significantly decreased in Scer\GAL4^elav.PLu>caz^VDRC.cUa larvae compared with controls.

Compared with wild-type, the average number of synaptic boutons per motor neuron is also significantly decreased as a result of Scer\GAL4^elav.PLu>caz^VDRC.cUa-expression.

(Azuma et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PS, caz^VDRC.cUa has visible | adult stage phenotype, enhanceable by Scer\GAL4^GMR.PS/lncRNA:Hsrω^EP93D

(Lo Piccolo and Yamaguchi, 2017)

Scer\GAL4^elav.PLu, caz^VDRC.cUa has abnormal locomotor behavior | progressive phenotype, enhanceable by TER94[+]/TER94^k15502

(Azuma et al., 2014)

Scer\GAL4^elav.PLu, caz^VDRC.cUa has abnormal neuroanatomy phenotype, enhanceable by TER94[+]/TER94^k15502

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has abnormal neuroanatomy phenotype, enhanceable by TER94[+]/TER94^k15502

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has abnormal neuroanatomy phenotype, enhanceable by TER94⁰³⁷⁷⁵/TER94[+]

(Azuma et al., 2014)

NOT Enhanced by

Statement

Reference

Scer\GAL4^elav.PLu, caz^VDRC.cUa has abnormal locomotor behavior | third instar larval stage phenotype, non-enhanceable by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^elav.PLu

(Lo Piccolo and Yamaguchi, 2017)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PS, caz^VDRC.cUa has visible | adult stage phenotype, suppressible by Scer\GAL4^GMR.PS/lncRNA:Hsrω^RNAi.Sym.UAS

(Lo Piccolo and Yamaguchi, 2017)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^GMR.PS/TER94^UAS.cHa

(Azuma et al., 2014)

Scer\GAL4^elav.PLu, caz^VDRC.cUa has abnormal locomotor behavior | progressive phenotype, suppressible by Scer\GAL4^elav.PLu/TER94^UAS.cHa

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^elav.PLu/TER94^UAS.cHa

(Azuma et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav.PLu, caz^VDRC.cUa has abnormal locomotor behavior | third instar larval stage phenotype, non-suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^elav.PLu

(Lo Piccolo and Yamaguchi, 2017)

Enhancer of

Statement

Reference

Scer\GAL4^GMR.PS/caz^VDRC.cUa is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PS, lncRNA:Hsrω^EP93D

(Lo Piccolo and Yamaguchi, 2017)

NOT Enhancer of

Statement

Reference

caz^VDRC.cUa/Scer\GAL4^elav.PLu is a non-enhancer of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4^elav.PLu, lncRNA:Hsrω^RNAi.Sym.UAS

(Lo Piccolo and Yamaguchi, 2017)

NOT Suppressor of

Statement

Reference

caz^VDRC.cUa/Scer\GAL4^elav.PLu is a non-suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4^elav.PLu, lncRNA:Hsrω^RNAi.Sym.UAS

(Lo Piccolo and Yamaguchi, 2017)

Other

Statement

Reference

+/Df(2R)X1, Scer\GAL4^GMR.PS, caz^VDRC.cUa has lethal - all die before end of P-stage phenotype

(Azuma et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PS, caz^VDRC.cUa has eye phenotype, enhanceable by Scer\GAL4^GMR.PS/lncRNA:Hsrω^EP93D

(Lo Piccolo and Yamaguchi, 2017)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has ommatidium | adult stage phenotype, enhanceable by Scer\GAL4^GMR.PS/lncRNA:Hsrω^EP93D

(Lo Piccolo and Yamaguchi, 2017)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has eye phenotype, enhanceable by TER94[+]/TER94^k15502

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has ommatidium phenotype, enhanceable by TER94[+]/TER94^k15502

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has interommatidial bristle phenotype, enhanceable by TER94[+]/TER94^k15502

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has eye phenotype, enhanceable by TER94⁰³⁷⁷⁵/TER94[+]

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has ommatidium phenotype, enhanceable by TER94⁰³⁷⁷⁵/TER94[+]

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has interommatidial bristle phenotype, enhanceable by TER94⁰³⁷⁷⁵/TER94[+]

(Azuma et al., 2014)

Scer\GAL4^elav.PLu, caz^VDRC.cUa has embryonic/larval motor neuron | larval stage phenotype, enhanceable by TER94[+]/TER94^k15502

(Azuma et al., 2014)

Scer\GAL4^elav.PLu, caz^VDRC.cUa has NMJ bouton | larval stage phenotype, enhanceable by TER94[+]/TER94^k15502

(Azuma et al., 2014)

NOT Enhanced by

Statement

Reference

Scer\GAL4^elav.PLu, caz^VDRC.cUa has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^elav.PLu

(Lo Piccolo and Yamaguchi, 2017)

Scer\GAL4^elav.PLu, caz^VDRC.cUa has NMJ bouton | third instar larval stage phenotype, non-enhanceable by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^elav.PLu

(Lo Piccolo and Yamaguchi, 2017)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PS, caz^VDRC.cUa has eye phenotype, suppressible by Scer\GAL4^GMR.PS/lncRNA:Hsrω^RNAi.Sym.UAS

(Lo Piccolo and Yamaguchi, 2017)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has ommatidium | adult stage phenotype, suppressible by Scer\GAL4^GMR.PS/lncRNA:Hsrω^RNAi.Sym.UAS

(Lo Piccolo and Yamaguchi, 2017)

Scer\GAL4^elav.PLu, caz^VDRC.cUa has NMJ bouton | larval stage phenotype, suppressible by Scer\GAL4^elav.PLu/TER94^UAS.cHa

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has eye phenotype, suppressible by Scer\GAL4^GMR.PS/TER94^UAS.cHa

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has ommatidium phenotype, suppressible by Scer\GAL4^GMR.PS/TER94^UAS.cHa

(Azuma et al., 2014)

Scer\GAL4^GMR.PS, caz^VDRC.cUa has interommatidial bristle phenotype, suppressible by Scer\GAL4^GMR.PS/TER94^UAS.cHa

(Azuma et al., 2014)

Scer\GAL4^elav.PLu, caz^VDRC.cUa has embryonic/larval motor neuron | larval stage phenotype, suppressible by Scer\GAL4^elav.PLu/TER94^UAS.cHa

(Azuma et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav.PLu, caz^VDRC.cUa has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^elav.PLu

(Lo Piccolo and Yamaguchi, 2017)

Scer\GAL4^elav.PLu, caz^VDRC.cUa has NMJ bouton | third instar larval stage phenotype, non-suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^elav.PLu

(Lo Piccolo and Yamaguchi, 2017)

Enhancer of

Statement

Reference

Scer\GAL4^GMR.PS/caz^VDRC.cUa is an enhancer of eye phenotype of Scer\GAL4^GMR.PS, lncRNA:Hsrω^EP93D

(Lo Piccolo and Yamaguchi, 2017)

Scer\GAL4^GMR.PS/caz^VDRC.cUa is an enhancer of ommatidium | adult stage phenotype of Scer\GAL4^GMR.PS, lncRNA:Hsrω^EP93D

(Lo Piccolo and Yamaguchi, 2017)

NOT Enhancer of

Statement

Reference

caz^VDRC.cUa/Scer\GAL4^elav.PLu is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Scer\GAL4^elav.PLu, lncRNA:Hsrω^RNAi.Sym.UAS

(Lo Piccolo and Yamaguchi, 2017)

caz^VDRC.cUa/Scer\GAL4^elav.PLu is a non-enhancer of NMJ bouton | third instar larval stage phenotype of Scer\GAL4^elav.PLu, lncRNA:Hsrω^RNAi.Sym.UAS

(Lo Piccolo and Yamaguchi, 2017)

NOT Suppressor of

Statement

Reference

caz^VDRC.cUa/Scer\GAL4^elav.PLu is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Scer\GAL4^elav.PLu, lncRNA:Hsrω^RNAi.Sym.UAS

(Lo Piccolo and Yamaguchi, 2017)

caz^VDRC.cUa/Scer\GAL4^elav.PLu is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Scer\GAL4^elav.PLu, lncRNA:Hsrω^RNAi.Sym.UAS

(Lo Piccolo and Yamaguchi, 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The eye morphology defects (rough appearance with fused ommatidia) characteristic for flies expressing caz^VDRC.cUa under the control of Scer\GAL4^GMR.PS can be rescued by co-expression of Hsrω^{dsRNA.Sym.Scer\UAS} and further worsened by co-expression of Hsrω^EP93D (which on its own also causes eye defects).

Third instar larvae simultaneously expressing both Hsrω^{dsRNA.Sym.Scer\UAS} and caz^VDRC.cUa under the control of Scer\GAL4^elav.PLu display crawling ability deficiency as well as morphological defects in the neuromuscular junctions (reduced total synaptic branch length and number of boutons) but the severity of each of these defects is not significantly different from either of the single knockdowns.

(Lo Piccolo and Yamaguchi, 2017)

Heterozygous TER94^k15502 enhances the rough-eye phenotype induced by Scer\GAL4^GMR.PS>caz^VDRC.cUa.

Heterozygous TER94⁰³⁷⁷⁵ enhances the rough-eye phenotype induced by Scer\GAL4^GMR.PS>caz^VDRC.cUa.

Expression of Scer\GAL4^GMR.PS>caz^VDRC.cUa in the presence of heterozygous Df(2R)X1 results in lethality at the pupal stage.

Co-expression of TER94^Scer\UAS.cHa suppresses the rough-eye phenotype induced by Scer\GAL4^GMR.PS>caz^VDRC.cUa.

Flies carrying TER94^k15502 and Scer\GAL4^elav.PLu>caz^VDRC.cUa have significantly worse locomotive ability than do flies with Scer\GAL4^elav.PLu>caz^VDRC.cUa alone.

Flies co-expressing TER94^Scer\UAS.cHa have significantly better climbing ability than do flies expressing Scer\GAL4^elav.PLu>caz^VDRC.cUa alone.

The decreased branch length phenotype caused by Scer\GAL4^elav.PLu>caz^VDRC.cUa in motor neurons is significantly enhanced by TER94^k15502/+.

The decrease in the number of synaptic boutons in Scer\GAL4^elav.PLu>caz^VDRC.cUa-expressing larvae is significantly enhanced by TER94^k15502/+. However, there is significant differences in the size of synaptic boutons among these genotypes.

Total branch length phenotype caused by Scer\GAL4^elav.PLu>caz^VDRC.cUa in motor neurons is significantly suppressed by the co-expression of TER94^Scer\UAS.cHa. The number of synaptic boutons is also significantly increased in Scer\GAL4^elav.PLu>caz^VDRC.cUa larvae co-expressing TER94^Scer\UAS.cHa compared with larvae expressing Scer\GAL4^elav.PLu>caz^VDRC.cUa alone.

(Azuma et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

caz^VDRC.cUa

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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