FlyBase Allele Report: Dmel\hiw[ΔN]

General Information

Symbol

Dmel\hiw^ΔN

Species

D. melanogaster

Name

FlyBase ID

FBal0296663

Feature type

allele

Associated gene

Dmel\hiw

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Collins et al., 2006, Wu et al., 2005)

Progenitor genotype

hiw^BG02015

(Wu et al., 2005)

Cytology

Description

The N-terminus of hiw is deleted due to imprecise excision of P{GT1}hiw^BG02015, removing the genomic region encoding the first 2784 amino acids of hiw.

(Wu et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of brain disease

CEA

(Hill et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Loreto et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | adult stage

(Neukomm et al., 2017)

axon | somatic clone

(Hsu et al., 2021)

axon | third instar larval stage | conditional

(Hao et al., 2019)

bouton

(Mozer and Sandstrom, 2012)

embryonic/larval motor neuron | third instar larval stage | conditional

(Hao et al., 2019)

embryonic/larval neuromuscular junction

(Tian et al., 2011)

embryonic/larval neuromuscular junction | larval stage

(Brace et al., 2014)

embryonic/larval neuromuscular junction | third instar larval stage

(Russo and DiAntonio, 2019)

indirect flight muscle motor neuron DLMN4

(Wu et al., 2005)

larval dorsal multidendritic neuron ddaC

(Kim et al., 2013)

larval segmental nerve | conditional

(Xiong et al., 2010)

neuromuscular junction

(Wu et al., 2005)

neuromuscular junction | larval stage

(Klinedinst et al., 2013)

NMJ bouton

(Tian et al., 2011)

NMJ bouton | increased number | third instar larval stage

(Russo and DiAntonio, 2019)

NMJ bouton | larval stage

(Brace et al., 2014, Klinedinst et al., 2013)

synapse

(Kim et al., 2013, Mozer and Sandstrom, 2012, Tian et al., 2011, Wu et al., 2005)

terminal bouton

(Wu et al., 2005)

wing nerve | somatic clone

(Hsu et al., 2021)

wing vein L1 (with hiw⁰⁵²)

(Neukomm et al., 2014)

Detailed Description

Statement

Reference

hiw^ΔN third instar larvae exhibit dramatic overgrowth of the neuromuscular junction, namely increased branching and increased number of presynaptic boutons, as compared to controls.

(Russo and DiAntonio, 2019)

hiw^ΔN mutants show defective injury-induced axon degeneration as the severed axons persist rather than be cleared away. Moreover, after an axotomy of mechanosensory neurons in the Johnston's organ, the persisting severed axons can still elicit grooming behavior upon optogenetic stimulation of the neurons.

(Neukomm et al., 2017)

Mutant larvae show a dramatic increase in the number of boutons at the neuromuscular junction compared to wild type.

Mutant larvae show a decrease in axonal degeneration after crush injury compared to wild type.

(Brace et al., 2014)

hiw⁰⁵²/hiw^ΔN has a strong protective effect on L1 vein neurons following axotomy. Whereas wild type axons undergo fragmentation, many severed but intact axons are observed. A similar protective effect is seen in axotomised olfactory receptor neurons.

(Neukomm et al., 2014)

Loss of hiw in hiw^ΔNterm mutants causes dramatic presynaptic overgrowth and ectopic synapses in C4 da neurons.

(Kim et al., 2013)

Mutant larvae show an increase in both branch and bouton number at the neuromuscular junction compared to controls.

(Klinedinst et al., 2013)

hiw^ΔN mutants display significantly enhanced aversive long term memory after one-session or two-session spaced training, but show normal long term memory after ten-session spaced training. Sensorimotor abilities are unchanged compared with controls.

(Huang et al., 2012)

hiw^ΔN mutants exhibit dramatic synaptic overgrowth yet reduced quantal content.

(Mozer and Sandstrom, 2012)

hiw^ΔN mutant neuromuscular junctions exhibit a decrease in bouton size compared to wild-type and a concurrent increase in the number of boutons (compared to wild-type).

(Tian et al., 2011)

Axonal sprouting of the injured axon after a crush injury to the segmental nerve in third instar larvae is accelerated approximately 5 hours in mutant animals compared to controls.

(Xiong et al., 2010)

Morphological analysis at the neuromuscular junction of hiw^ΔNterm mutants reveals a dramatic synaptic overgrowth phenotype apparent at every type I synapse.

Using the MN4-Ib motoneuron as a model, hiw^ΔNterm mutants exhibit a fivefold increase in the numbers of both synaptic bouton and branch points when compared with wild-type neuromuscular junctions. The synaptic span is also incrase from approximately 15% in wild-type to approximately 40%. Although there is an increase in the number of synaptic boutons, there is an approximate 70% decrease in the average bouton size compared to wild-type.

(Wu et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

hiw^ΔN has abnormal neuroanatomy phenotype, suppressible by Dscam1¹⁸

(Kim et al., 2013)

hiw^ΔN has abnormal neuroanatomy phenotype, suppressible by Fmr1^UAS.cZa/Scer\GAL4^ppk.PG

(Kim et al., 2013)

hiw^ΔN has abnormal neuroanatomy | larval stage phenotype, suppressible by Aplip1^ex

(Klinedinst et al., 2013)

Suppressor of

Statement

Reference

hiw^ΔN is a suppressor | partially of abnormal neuroanatomy | recessive | third instar larval stage | conditional phenotype of raw^dcp-1

(Hao et al., 2019)

hiw^ΔN is a suppressor of abnormal neuroanatomy | larval stage phenotype of Aplip1^ex

(Klinedinst et al., 2013)

NOT Suppressor of

Statement

Reference

hiw^ΔN is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

hiw^ΔN is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

hiw^ΔN has larval dorsal multidendritic neuron ddaC phenotype, suppressible by Dscam1¹⁸

(Kim et al., 2013)

hiw^ΔN has synapse phenotype, suppressible by Dscam1¹⁸

(Kim et al., 2013)

hiw^ΔN has larval dorsal multidendritic neuron ddaC phenotype, suppressible by Fmr1^UAS.cZa/Scer\GAL4^ppk.PG

(Kim et al., 2013)

hiw^ΔN has synapse phenotype, suppressible by Fmr1^UAS.cZa/Scer\GAL4^ppk.PG

(Kim et al., 2013)

hiw^ΔN has NMJ bouton | larval stage phenotype, suppressible by Aplip1^ex

(Klinedinst et al., 2013)

hiw^ΔN has neuromuscular junction | larval stage phenotype, suppressible by Aplip1^ex

(Klinedinst et al., 2013)

NOT suppressed by

Statement

Reference

hiw^ΔN has embryonic/larval neuromuscular junction phenotype, non-suppressible by Scer\GAL4^elav-C155/Rae1^UAS.Tag:TAP

(Tian et al., 2011)

hiw^ΔN has synapse phenotype, non-suppressible by Scer\GAL4^elav-C155/Rae1^UAS.Tag:TAP

(Tian et al., 2011)

hiw^ΔN has NMJ bouton phenotype, non-suppressible by Scer\GAL4^elav-C155/Rae1^UAS.Tag:TAP

(Tian et al., 2011)

Suppressor of

Statement

Reference

hiw^ΔN is a suppressor | partially of axon | third instar larval stage | conditional phenotype of raw^dcp-1

(Hao et al., 2019)

hiw^ΔN is a suppressor | partially of embryonic/larval motor neuron | third instar larval stage | conditional phenotype of raw^dcp-1

(Hao et al., 2019)

hiw^ΔN is a suppressor of NMJ bouton | larval stage phenotype of Aplip1^ex

(Klinedinst et al., 2013)

NOT Suppressor of

Statement

Reference

hiw^ΔN is a non-suppressor of sensory neuron | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

hiw^ΔN is a non-suppressor of axon | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

hiw^ΔN is a non-suppressor of sensory neuron | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

hiw^ΔN is a non-suppressor of axon | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

hiw^ΔN is a non-suppressor of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

raw^dcp-1 homozygotes show protection against (injury-induced) Wallerian degeneration in third instar larval motor neurons, which is considerably suppressed by hiw^ΔN.

(Hao et al., 2019)

MARCM clones in sensory neurons in the adult wing expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^VGlut-OK371 undergo spontaneous cell body and axon degeneration which cannot be suppressed by combination hiw^ΔN.

The axon and cell body degeneration in sensory neuron clones in the adult wing expressing Nmnat^GD8082 under the control of Scer\GAL4^VGlut-OK371 is only marginally improved by hiw^ΔN.

(Neukomm et al., 2017)

The dramatic overgrowth of presynaptic arbors found in hiw^ΔNterm mutant C4 da neurons is completely abolished in a Dscam1¹⁸ MARCM clone background.

The dramatic overgrowth of presynaptic arbors found in hiw^ΔNterm mutant C4 da neurons is completely abolished by expression of Fmr1^Scer\UAS.cZa under the control of Scer\GAL4^ppk.PG.

(Kim et al., 2013)

Aplip1^ex suppresses the increase in branch and bouton number at the neuromuscular junction which is seen in hiw^ΔNterm larvae. hiw^ΔNterm suppresses the increase in maximum bouton diameter which is seen at the neuromuscular junction in Aplip1^ex larvae.

(Klinedinst et al., 2013)

Presynaptic expression of Rae1^{Scer\UAS.T:Zzzz\TAP} under the control of Scer\GAL4^elav-C155 fails to rescue the bouton number increase found in hiw^ΔN mutant synapses.

(Tian et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51637

w^* hiw^ΔN

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Hiw^DN

(Chitre et al., 2024)

Hiw^ΔN

(Loreto et al., 2020, Cai et al., 2019)

hiw^ΔN

(Kim et al., 2024, Llobet Rosell et al., 2022, Singh et al., 2022, Hsu et al., 2021, Hill et al., 2020, Liu et al., 2020, Hao et al., 2019, Li et al., 2017, Neukomm et al., 2017, Brace et al., 2014, Neukomm et al., 2014, Klinedinst et al., 2013, Huang et al., 2012, Mozer and Sandstrom, 2012, Shin and Diantonio, 2011, Tian et al., 2011, Xiong et al., 2010, Collins et al., 2006, Wu et al., 2005)

hiw^ΔNterm

(Kim et al., 2013)

hiwΔN

(Russo and DiAntonio, 2019)

Name Synonyms

Secondary FlyBase IDs

FBal0215864
FBal0194607

References (25)

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