FB2025_02 , released April 17, 2025
Allele: Dmel\E(spl)m8-HLHUAS.ORF.GW.Tag:HA
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General Information
Symbol
Dmel\E(spl)m8-HLHUAS.ORF.GW.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0296979
Feature type
allele
Associated gene
Dmel\E(spl)m8-HLH
Associated Insertion(s)
Carried in Construct
M{UAS-E(spl)m8-HLH.ORF.3xHA.GW}
Key Links
Transgenic product class
wild_type
(Bischof and FlyORF project members, 2014.6.20)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bischof and FlyORF project members, 2014.6.20)
Mutagen
in vitro construct
(Bischof and FlyORF project members, 2014.6.20)
Progenitor genotype
Carried in construct
M{UAS-E(spl)m8-HLH.ORF.3xHA.GW}
Cytology
Description

UAS regulatory sequences drive expression of a full-length E(spl)m8-HLH open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Bischof and FlyORF project members, 2014.6.20)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
E(spl)m8-HLH
Tagged with
Tag:HA
Also carries
FRT2
FRT5
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of E(spl)m8-HLHScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in male flies with weak wing size and patterning defects.

      Expression of E(spl)m8-HLHScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in female flies with weak wing size and patterning defects.

      (Schertel et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      E(spl)m8-HLHScer\UAS.ORF.GW.T:Ivir\HA1
      E(spl)m8-HLHUAS.ORF.GW.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (3)