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General Information
Symbol
Dmel\ermUAS.ORF.GW.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0296995
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a full-length erm open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ermUAS.ORF.GW.Tag:HA under the control of Scer\GAL4elav-C155 increases mitotic proliferation of neuroblasts and their daughter cells in the ventral nerve cord during late embryogenesis, compared to controls; expression under the control of Scer\GAL4vg.int2.1 in the larval wing disc has only a limited effect on the number of neuroblasts and their daughter cells, compared to controls.

Expression of ermScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in female flies with severe wing size and patterning defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Enhancer of
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Statement
Reference
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of tllUAS.cKa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA and Doc2UAS.cRa under the control of Scer\GAL4elav-C155 significantly increases total cell number and the number of neuroblasts in the late embryonic ventral nerve cord, compared to controls; expression under the control of Scer\GAL4wg.PU results in a trend towards increased symmetric (abnormal) neuroblast division and significantly increased asymmetric (normal) daughter (ganglion mother cell) division as well as a significant increase in the number of cells in the NB5-6 lineage, compared to controls; expression under the control of Scer\GAL4pros.PMG significantly increases the number of neuroblasts and daughters in S-phase, with no effect on cell cycle speed, compared to controls; expression under the control of Scer\GAL4da.PU increases the number of mitotic cells, neuroblasts, daughters and neurons, and reduces the number of glia, with a thickening of the CNS, compared to controls.

Expression of tllUAS.cKa and ermUAS.ORF.GW.Tag:HA under the control of Scer\GAL4elav-C155 significantly increases the number of neuroblasts in the late embryonic ventral nerve cord, compared to controls; expression under the control of Scer\GAL4wg.PU results in a significant increase in symmetric (abnormal) neuroblast division and asymmetric (normal) daughter (ganglion mother cell) division as well as a significant increase in the number of cells in the NB5-6 lineage, compared to controls; expression under the control of Scer\GAL4pros.PMG significantly increases the number of neuroblasts and daughters in S-phase, with no effect on cell cycle speed, compared to controls; expression under the control of Scer\GAL4da.PU increases the number of mitotic cells, neuroblasts, neuroblast daughters, neurons and glia, compared to controls; expression under the control of Scer\GAL4[vg.int2.1] results in the formation of neurons and glia in the larval wing disc, these are not seen in controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ermScer\UAS.ORF.GW.T:Ivir\HA1
ermUAS.ORF.GW.Tag:HA
Name Synonyms
Secondary FlyBase IDs
    References (3)