When expressed with "MTD-GAL4" (which comprises Scer\GAL4^nos.PG, Scer\GAL4^{nos.UTR.T:Hsim\VP16} and Scer\GAL4^{otu.T:Hsim\VP16}), and females have been provided with a protein source of wet yeast, then females are fertile similar to wild type. However, when similarly expressed in females that were starved for amino acids, then there is a marked increase in the number of degenerating (apoptotic) young egg chambers, compared to controls. Rapamycin-treated mutant, starved females show a marked decrease in the percentage of dying young egg chambers.

In contrast to wild type, the number of eggs laid after 7-days of amino acid starvation and the near shutdown of oogenesis does not rapidly increase in "MTD-GAL4" Nprl2^{dsRNA.shRNA.Scer\UAS.P\T} females after the reintroduction of a protein source. Consistent with this, recovering ovarioles often contain numerous degenerating egg chambers. However, rapamycin-treated mutant females show oogenesis recovery kinetics similar to controls.

Nprl2^RNAi.UAS.V22, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of ovary phenotype of Akt^HMS00007, Scer\GAL4^{VP16.nanos.UTR}