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General Information
Symbol
Dmel\Nprl2dsRNA.shRNA.UAS.V22
Species
D. melanogaster
Name
FlyBase ID
FBal0298084
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of shRNA sequences targeting the Nprl2 transcript (the pVALIUM22 vector was used).
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
When expressed with "MTD-GAL4" (which comprises Scer\GAL4nos.PG, Scer\GAL4nos.UTR.T:Hsim\VP16 and Scer\GAL4otu.T:Hsim\VP16), and females have been provided with a protein source of wet yeast, then females are fertile similar to wild type. However, when similarly expressed in females that were starved for amino acids, then there is a marked increase in the number of degenerating (apoptotic) young egg chambers, compared to controls. Rapamycin-treated mutant, starved females show a marked decrease in the percentage of dying young egg chambers. In contrast to wild type, the number of eggs laid after 7-days of amino acid starvation and the near shutdown of oogenesis does not rapidly increase in "MTD-GAL4" Nprl2dsRNA.shRNA.Scer\UAS.P\T females after the reintroduction of a protein source. Consistent with this, recovering ovarioles often contain numerous degenerating egg chambers. However, rapamycin-treated mutant females show oogenesis recovery kinetics similar to controls.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Co-expression of Nprl2dsRNA.shRNA.Scer\UAS.P\T does not rescue the small ovary phenotype resulting from Scer\GAL4nos.UTR.T:Hsim\VP16-mediated expression of Akt1HMS00007.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Nprl2dsRNA.shRNA.Scer\UAS.P\T
Nprl2dsRNA.shRNA.UAS.V22
Name Synonyms
Secondary FlyBase IDs
    References (1)