FB2025_01 , released February 20, 2025
Allele: Dmel\Nprl3RNAi.UAS.V20
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General Information
Symbol
Dmel\Nprl3RNAi.UAS.V20
Species
D. melanogaster
Name
FlyBase ID
FBal0298086
Feature type
allele
Associated gene
Dmel\Nprl3
Associated Insertion(s)
Carried in Construct
P{UAS-Nprl3.RNAi.V20}
Key Links
Transgenic product class
RNAi_reagent
(Wei and Lilly, 2014)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Wei and Lilly, 2014)
Mutagen
in vitro construct
(Wei and Lilly, 2014)
Progenitor genotype
Carried in construct
P{UAS-Nprl3.RNAi.V20}
Cytology
Description

UAS regulates expression of shRNA sequences targeting the Nprl3 transcript (the pVALIUM20 vector was used)

(Wei and Lilly, 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Nprl3
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4VP16.nanos.UTR/Nprl3RNAi.UAS.V20 is a non-suppressor of ovary phenotype of Nup44AΔ15

      (Cai et al., 2016)

      Scer\GAL4VP16.nanos.UTR/Nprl3RNAi.UAS.V20 is a non-suppressor of ovary phenotype of mio2

      (Cai et al., 2016)

      Scer\GAL4VP16.nanos.UTR/Nprl3RNAi.UAS.V20 is a non-suppressor of autolysosome phenotype of Wdr241

      (Cai et al., 2016)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Nprl3dsRNA.shRNA.Scer\UAS driven by Scer\GAL4nos.UTR.T:Hsim\VP16 significantly partially suppresses the small ovaries and reduced fertility seen in Wdr241/Wdr241 females. Expression of Nprl3dsRNA.shRNA.Scer\UAS driven by Scer\GAL4hs.PU significantly partially suppresses the reduced body size (measured body weight) seen in Wdr241/Wdr241 flies. Expression of Nprl3dsRNA.shRNA.Scer\UAS driven by Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the egg chamber growth defect but not the inappropriate accumulation of autolysosomes seen in Wdr241/Wdr241 flies (though autophagy activation is reduced). Expression of Nprl3dsRNA.shRNA.Scer\UAS driven by Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress accumulation of acidic puncta in mio2/mio2 or Nup44AΔ15/Nup44AΔ15 ovaries.

      (Cai et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Nprl3RNAi.UAS.V20
      Nprl3dsRNA.shRNA.Scer\UAS
      Nprl3dsRNA.shRNA.UAS.V20
      nprl3RNAi
      (Cai et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)