When expressed with "MTD-GAL4" (which comprises Scer\GAL4nos.PG, Scer\GAL4nos.UTR.T:Hsim\VP16 and Scer\GAL4otu.T:Hsim\VP16), and females have been provided with a protein source of wet yeast, then females are fertile similar to wild type. However, when similarly expressed in females that were starved for amino acids, then there is a marked increase in the number of degenerating (apoptotic) young egg chambers, compared to controls. Rapamycin-treated mutant, starved females show a marked decrease in the percentage of dying young egg chambers.
In contrast to wild type, the number of eggs laid after 7-days of amino acid starvation and the near shutdown of oogenesis does not rapidly increase in "MTD-GAL4" Nprl3dsRNA.shRNA.Scer\UAS.P\T females after the reintroduction of a protein source. Consistent with this, recovering ovarioles often contain numerous degenerating egg chambers. However, rapamycin-treated mutant females show oogenesis recovery kinetics similar to controls.