FB2025_01 , released February 20, 2025
Allele: Dmel\mnbUAS.Tag:HA
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General Information
Symbol
Dmel\mnbUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0298820
Feature type
allele
Associated gene
Dmel\mnb
Associated Insertion(s)
Carried in Construct
P{UAS-mnb.HA}
Key Links
Transgenic product class
wild_type
(Chen et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Chen et al., 2014)
Mutagen
in vitro construct
(Chen et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-mnb.HA}
Cytology
Description

UAS regulatory sequences drive expression of the mnb F isoform coding region tagged with Tag:HA.

(Chen et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mnb
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The NMJs of larvae expressing mnbScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS show an increased number of synaptic boutons compared to controls, and these boutons are smaller than in controls.

      Expression of mnbScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS has no effect on synaptic transmission or responses to high-frequency stimulation compared with controls.

      Larvae expressing mnbScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS exhibit enhanced synaptic vesicle endocytosis: the level of membrane uptake (dye internalisation) in mutant synaptic boutons in response to nerve stimulation is significantly increased compared to controls. When dye unloading occurs in response to a second stimulation, the mutant larvae also unload an increased amount of dye to controls.

      (Chen et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      mnbUAS.Tag:HA/Scer\GAL4nSyb.PS rescues mnb1

      (Chen et al., 2014)
      Comments

      Pre-synaptic expression of mnbScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS rescues the bouton and miniature excitatory postsynaptic potential (mEPSP) defects seen in mnb1 mutants. The synaptic vesicle endocytosis defects are also rescued.

      (Chen et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      mnbScer\UAS.T:Ivir\HA1
      mnbUAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)