FB2025_01 , released February 20, 2025
Allele: Dmel\cmbKO
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General Information
Symbol
Dmel\cmbKO
Species
D. melanogaster
Name
FlyBase ID
FBal0298847
Feature type
allele
Associated gene
Dmel\cmb
Associated Insertion(s)
TI{TI}cmbKO
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Fagan et al., 2014)
Mutagen
gene targeting by homologous recombination
(Fagan et al., 2014)
Progenitor genotype
Associated Insertion(s)
TI{TI}cmbKO
Cytology
Description

1023bp of cmb exon 3, including the start codon and roughly half of exon 4 of cmb-RB (equivalent to exon 4 and half of exon 5 of the other isoforms), is replaced with a loxP cassette containing a wGMR.PH marker.

(Fagan et al., 2014)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:13,444,211..13,445,233 [+]
Comment:

Inferred boundaries of 1023 bp region in cmb that has been replaced with a w marker.

(Fagan et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      cmbKO heterozygous males are fertile and do not exhibit any defects in spermiogenesis compared to controls.

      In cmbKO homozygotes the male germline cells proceed through spermatogenesis normally until the individualization stage; individualization is initiated (with individualization complexes (ICs) forming and caspases being activated) but the advancement of spermatid individualization is highly perturbed (spermatids are still surrounded by cytoplasm, minor mitochondrial derivatives are enlarged, late ICs with lower actin levels, scattered actin cones have structural defects and waste bags do not form); the number of abnormal ICs are significantly higher; and the seminal vesicles lack mature sperm when compared to controls.

      (Steinhauer et al., 2019)

      cmbKO flies are viable with no gross anatomical defects. Trichome polarity in the wing is normal, and there is no 'multiple wing hair' phenotype. Similarly, adult eye are normal with no polarity defects.

      (Fagan et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      cmbKO has male sterile | recessive phenotype, non-suppressible by mwh1

      (Steinhauer et al., 2019)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      cmbKO is a suppressor of wing hair | increased number phenotype of mwh6/mwh1

      (Fagan et al., 2014)

      cmbKO is a suppressor of wing hair | increased number phenotype of mwh6

      (Fagan et al., 2014)

      cmbKO is a suppressor of wing hair | increased number phenotype of mwh1

      (Fagan et al., 2014)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Homozygosity for cmbKO significantly suppresses the multiple hair cell phenotype of mwh1, mwh6 and mwh1/mwh6 wings.

      (Fagan et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      cmbKO
      (Espinoza and Berg, 2020, Steinhauer et al., 2019, Fagan et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)