FB2025_02 , released April 17, 2025
Allele: Dmel\TBPHF-L150-152.UAS
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General Information
Symbol
Dmel\TBPHF-L150-152.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0299628
Feature type
allele
Associated gene
Dmel\TBPH
Associated Insertion(s)
Carried in Construct
M{UAS-TBPH.F-L150-152}
Also Known As
UAS-TBPHF/L
Key Links
Transgenic product class
missense_variant
(Godena et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Godena et al., 2011)
Mutagen
in vitro construct
(Godena et al., 2011)
Progenitor genotype
Carried in construct
M{UAS-TBPH.F-L150-152}
Cytology
Description

UAS regulatory sequences drive expression of a mutant form of TBPH in which the phenylalanine at amino acids 150 and 152 have been mutated to leucine.

(Godena et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
TBPH
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing TBPHF-L150-152.Scer\UAS under the control of Scer\GAL4elav.PU, and restricted to the adult stage using Scer\GAL80ts.αTub84B, do not exhibit climbing defects compared to controls.

      (Romano et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      TBPHF-L150-152.UAS/Scer\GAL4Toll-6-D42 partially rescues TBPHΔ23

      (Godena et al., 2011)
      Fails to rescue

      Scer\GAL4elav.Switch.PO/TBPHF-L150-152.UAS fails to rescue TBPHΔ23

      (Romano et al., 2014)
      Comments

      Expression of TBPHF-L150-152.Scer\UAS under the control of Scer\GAL4elav.Switch.PO (fed 0.05 or 0.25ul RU486 throughout the larval stages) is not sufficient to rescue the larval locomotion, NMJ growth and cytoskeletal defects in synaptic boutons seen in TBPHΔ23 mutants.

      (Romano et al., 2014)

      Expression of TBPHF-L150-152.Scer\UAS under the control of Scer\GAL4D42 fails to rescue the reduction in bouton number and terminal branches seen in muscles 6 and 7 of homozygous TBPHΔ23 third instar larvae. The defects in microtubule stability are also not rescued. Fly motility, including larval movement, adult flies walking and climbing, is partially rescued.

      (Godena et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TBPHF-L150-152.Scer\UAS
      TBPHF-L150-152.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (5)