FB2019_05, released Oct 15, 2019

Allele: Dmel\vtdNC.UASp.Tag:MYC

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General Information
Symbol
Dmel\vtdNC.UASp.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0299646
Feature type
allele
Associated gene
Dmel\vtd
Associated Insertion(s)
Carried in Construct
P{UASp-Rad21.NC.myc}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Urban et al., 2014)
in vitro construct - coding region fusion
(Urban et al., 2014)
in vitro construct - amino acid replacement
(Urban et al., 2014)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UASp-Rad21.NC.myc}
      Cytology
      Nature of the lesion
      Statement
      Reference
      UASp regulatory sequences drive expression of a mutant form of vtd which contains the amino acid replacements R175A and R474A. These mutations are within the two EXXR separase consensus sequences present in the protein and should result in a separase-resistant variant of vtd. The protein is tagged at the C-terminal end with 10 copies of Tag:MYC.
      (Urban et al., 2014)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASp
      Encoded product / tool
      vtd
      Tagged with
      Tag:MYC
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      Females expressing vtdNC.Scer\UAS.P\T.T:Hsap\MYC under the simultaneous control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 are almost completely sterile. The meiotic divisions appear to be normal, but mitotic divisions in early embryos derived from these females are severely affected. Many embryos contain only a single DNA mass. Prominent anaphase bridges and chromosome stretching are seen.
      (Urban et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      vtdNC.Scer\UAS.P\T.T:Hsap\MYC
      vtdNC.UASp.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (1)