Deletion of 5 amino acids (GHSLG) in the enzyme active site created on 31 kb PAPLA1 rescue genomic fragment by site-directed mutagenesis.
A 31-kb genomic fragment modified to carry an active site mutation in PAPLA1. The residues corresponding to the sequence GHSLG at 1682-1686 in the PAPLA1 polypeptide are deleted.
male sterile, with PAPLA1KO
PAPLA1Δ1682-1686 fails to rescue PAPLA1KO
Transgenic lines encoding PAPLA1Δ1682-1686 fail to rescue the male sterile phenotype of PAPLA1KO.