FB2025_01 , released February 20, 2025
Allele: Dmel\ReepAΔ275
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General Information
Symbol
Dmel\ReepAΔ275
Species
D. melanogaster
Name
FlyBase ID
FBal0301162
Feature type
allele
Associated gene
Dmel\ReepA
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Appocher et al., 2014)

amorphic allele - molecular evidence

(Appocher et al., 2014)
Mutagen
Delta2-3 transposase
(Appocher et al., 2014)
Progenitor genotype
P{SUPor-P}ReepAKG05246
(Appocher et al., 2014)
Cytology
Description

Imprecise excision of P{SUPor-P}Reep1KG05246 resulting in a 3.0 kb genomic deletion removing part of the Reep1 locus.

(Appocher et al., 2014)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2R:22,925,203..22,928,223 [+]
Comment:

A ~3.0 kb deletion of part of ReepA resulting from the imprecise excision of P{SUPor-P}ReepAKG05246. The boundaries were reported as 2R:18812726-18815746 in release 5 coordinates.

(Appocher et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  tauopathy
      modeled by Hsap\MAPTUAS.2N4R.cAa
      (Appocher et al., 2014)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous Reep1Δ275 mutant flies are perfectly viable and fertile. No difference between the lifespan of the mutants and controls is detected. Df(2R)BSC784 does not modify these phenotypes. Heteroallelic combination of Reep1Δ275 and Reep1Δ258 fails to reveal any abnormalities in locomotory behaviour (evaluated in climbing assays).

      Homozygous Reep1Δ275 and Reep1Δ275/Df(2R)BSC784 mutants show lower resistance to high temperatures compared with wild-type.

      Compared with wild-type, homozygous Reep1Δ275 and Reep1Δ275/Df(2R)BSC784 mutant flies show increased mortality upon tunicamycin treatment when testing response to misfolded protein stress in the endoplasmic reticulum.

      (Appocher et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Reep1Scer\UAS.T:Zzzz\FLAG in postmitotic neurons using the pan-neuronal driver Scer\GAL4nSyb.PS can rescue the stress sensitivity phenotypes of Reep1Δ258/Reep1Δ275 and Reep1Δ275/Df(2R)BSC784 mutants.

      Reep1Δ258/Reep1Δ275 enhances the neurodegeneration in retinas resulting from the expression of Scer\GAL4GMR.PU>Hsap\MAPTScer\UAS.2N4R.cAa resulting in the formation of extensive necrotic areas, visible as black spots, over the eye surface.

      Overexpression of Reep1Scer\UAS.T:Zzzz\FLAG ameliorates the Scer\GAL4GMR.PU>Hsap\MAPTScer\UAS.2N4R.cAa-induced eye-degeneration in the Reep1Δ258/Reep1Δ275 genetic background.

      Overexpression of Hsap\REEP1Scer\UAS.T:Zzzz\FLAG ameliorates the Scer\GAL4GMR.PU>Hsap\MAPTScer\UAS.2N4R.cAa-induced eye-degeneration in the Reep1Δ258/Reep1Δ275 genetic background.

      Reep1Δ258/Reep1Δ275 strongly increases the pathological formation of amyloid aggregates in retinas expressing Scer\GAL4GMR.PU>Hsap\MAPTScer\UAS.2N4R.cAa as well as the incidence of ectopic actin-rich structures within these amyloid clusters.

      (Appocher et al., 2014)
      Complementation and Rescue Data
      Rescued by

      Df(2R)BSC784/ReepAΔ275 is rescued by ReepAUAS.Tag:FLAG/Scer\GAL4nSyb.PS

      (Appocher et al., 2014)

      ReepAΔ258/ReepAΔ275 is rescued by ReepAUAS.Tag:FLAG/Scer\GAL4nSyb.PS

      (Appocher et al., 2014)
      Comments

      Expression of Reep1Scer\UAS.T:Zzzz\FLAG in postmitotic neurons using the pan-neuronal driver Scer\GAL4nSyb.PS can rescue the stress sensitivity phenotypes of Reep1Δ258/Reep1Δ258 and Reep1Δ258/Reep1Δ275 mutants.

      (Appocher et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      D-reep1Δ275
      (Appocher et al., 2014)
      Reep1Δ275
      ReepAΔ275
      Name Synonyms
      Secondary FlyBase IDs
        References (1)