FlyBase Allele Report: Hsap\REEP1[UAS.Tag:FLAG]

General Information

Symbol

Hsap\REEP1^UAS.Tag:FLAG

Species

H. sapiens

Name

FlyBase ID

FBal0301164

Feature type

allele

Associated gene

Hsap\REEP1

Associated Insertion(s)

Carried in Construct

M{UAS-hREEP1.FLAG}

Key Links

Transgenic product class

cDNA

(Appocher et al., 2014)

wild_type

(Appocher et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Appocher et al., 2014)

wild_type

(Appocher et al., 2014)

Mutagen

in vitro construct

(Appocher et al., 2014)

Progenitor genotype

Carried in construct

M{UAS-hREEP1.FLAG}

Cytology

Description

UASt regulatory sequences drive expression of a Hsap\REEP1 cDNA fused with Tag:FLAG.

(Appocher et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\REEP1

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.2N4R.cAa, ReepA^Δ258, ReepA^Δ275

(Appocher et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\REEP1^UAS.Tag:FLAG/Scer\GAL4^nSyb.PS is a suppressor of abnormal heat stress response phenotype of ReepA^Δ258/Df(2R)BSC784

(Appocher et al., 2014)

Hsap\REEP1^UAS.Tag:FLAG/Scer\GAL4^nSyb.PS is a suppressor of abnormal stress response phenotype of ReepA^Δ258/Df(2R)BSC784

(Appocher et al., 2014)

Hsap\REEP1^UAS.Tag:FLAG/Scer\GAL4^nSyb.PS is a suppressor of abnormal heat stress response phenotype of ReepA^Δ258/ReepA^Δ275

(Appocher et al., 2014)

Hsap\REEP1^UAS.Tag:FLAG/Scer\GAL4^nSyb.PS is a suppressor of abnormal stress response phenotype of ReepA^Δ258/ReepA^Δ275

(Appocher et al., 2014)

Scer\GAL4^GMR.PU, Hsap\REEP1^UAS.Tag:FLAG is a suppressor of visible phenotype of Hsap\MAPT^UAS.2N4R.cAa, ReepA^Δ258/ReepA^Δ275, Scer\GAL4^GMR.PU

(Appocher et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PU, Hsap\REEP1^UAS.Tag:FLAG is a suppressor of eye phenotype of Hsap\MAPT^UAS.2N4R.cAa, ReepA^Δ258/ReepA^Δ275, Scer\GAL4^GMR.PU

(Appocher et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\REEP1^{Scer\UAS.T:Zzzz\FLAG} in postmitotic neurons using the pan-neuronal driver Scer\GAL4^nSyb.PS can suppress the heat-shock sensitivity phenotype of Reep1^Δ258 mutants.

Expression of Hsap\REEP1^{Scer\UAS.T:Zzzz\FLAG} in postmitotic neurons using the pan-neuronal driver Scer\GAL4^nSyb.PS can suppress the heat-shock sensitivity phenotype of Reep1^Δ275 mutants.

Overexpression of Hsap\REEP1^{Scer\UAS.T:Zzzz\FLAG} ameliorates the Scer\GAL4^GMR.PU>Hsap\MAPT^{Scer\UAS.2N4R.cAa}-induced eye-degeneration in the Reep1^Δ258/Reep1^Δ275 genetic background.

(Appocher et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93605

w^*; M{UAS-hREEP1.FLAG}ZH-86Fa/TM6C, Sb¹ Tb¹

Notes on Origin