FB2025_01 , released February 20, 2025
Allele: Dmel\bdlUAS.Tag:FLAG
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General Information
Symbol
Dmel\bdlUAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0301365
Feature type
allele
Associated gene
Dmel\bdl
Associated Insertion(s)
Carried in Construct
P{UAS-bdl.Flag}
Also Known As
UAS-bdl
Key Links
Transgenic product class
wild_type
(Cameron et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Cameron et al., 2013)
Mutagen
in vitro construct
(Cameron et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-bdl.Flag}
Cytology
Description

UASt regulatory sequences drive expression of bdl with a C-terminal Tag:FLAG tag.

(Cameron et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
bdl
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of bdlScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mz97 in third instar larvae leads to a significant decrease in the extension of wrapping glial processes in the lamina, as compared to wild type. These larvae also exhibit abnormal wrapping of photoreceptor axon bundles in the optic stalk as compared with wild type, with some bundles wrapped by multiple layers of glial processes.

      (Cameron et al., 2016)

      In wild-type, R7 and R8 rhabdomere axons in the eye form a regularly spaced columnar projection pattern and terminate in two separate synaptic layers M6 and M3, respectively. In flies overexpressing bdlScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU, R7 axonal terminals frequently stick with R7 terminals in neighbouring columns, and many R7 axons terminate abnormally at the R8 terminal layer (M3 layer).

      (Cameron et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of LarScer\UAS.cCa.T:Ivir\HA1 significantly suppresses the R7 mis-targeting phenotype in transgenic flies expressing Scer\GAL4GMR.PU>bdlScer\UAS.T:Zzzz\FLAG.

      (Cameron et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      bdlUAS.Tag:FLAG rescues bdlEX2

      (Cameron et al., 2016)
      Comments

      Expression of bdlScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mz97 in third instar larvae rescues the wrapping glial extension defects seen in bdlEX2/bdlEX2 mutants.

      (Cameron et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      bdlScer\UAS.T:Zzzz\FLAG
      bdlUAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (3)