FlyBase Allele Report: Dmel\p53[UAS.A.Tag:MYC]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Dmel\p53^{UAS.A.Tag:MYC}

General Information

Symbol

Dmel\p53^{UAS.A.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0301411

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{UAS-Myc-p53.A}

PBac{UAS-Myc-p53.A}

Key Links

Transgenic product class

(Mehrotra et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Mehrotra et al., 2008)

Mutagen

in vitro construct

(Mehrotra et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Myc-p53.A}

PBac{UAS-Myc-p53.A}

Cytology

Description

UAS regulatory sequences drive expression of the A isoform of p53 tagged at the N-terminal end with 6xTag:MYC.

(Zhang et al., 2014)

UAS regulatory sequences drive expression of p53 tagged at the N-terminal end with 6xTag:MYC.

(Mehrotra et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^fkh.PH

(Zhang et al., 2014)

increased cell death, with Scer\GAL4^hs.PU

(Mehrotra et al., 2008)

increased cell death | adult stage | heat sensitive, with Scer\GAL4^hs.PU

(Zhang et al., 2015)

increased cell death | third instar larval stage, with Scer\GAL4^en.PU

(Zhang et al., 2015)

increased cell death | third instar larval stage, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

lethal, with Scer\GAL4^Act5C.PU

(Zhang et al., 2015)

visible, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

eye disc | third instar larval stage, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

follicle cell | heat sensitive, with Scer\GAL4^hs.PU

(Zhang et al., 2015)

interommatidial bristle, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

ommatidium, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

wing disc | third instar larval stage, with Scer\GAL4^fkh.PH

(Zhang et al., 2014)

wing disc posterior compartment, with Scer\GAL4^en.PU

(Zhang et al., 2015)

Detailed Description

Statement

Reference

Flies expressing p53^{Scer\UAS.A.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU have rough eyes with fused and irregular ommatidia and bristles.

Compared with controls, expression of Scer\GAL4^GMR.PU>p53^{Scer\UAS.A.T:Hsap\MYC} results in an increase in the number of apoptotic cells behind the morphogenetic furrow in eye discs.

Expression of p53^{Scer\UAS.A.T:Hsap\MYC} in the ovary under the control of Scer\GAL4^hs.PU results in a significant increase in the number of apoptotic somatic follicle cells compared with controls.

(Zhang et al., 2015)

Expression of p53^{Scer\UAS.A.T:Hsap\MYC} under the control of Scer\GAL4^fkh.PH induces apoptosis in third instar larval wing disc, but less than is seen with p53^{Scer\UAS.A.T:Hsap\MYC}.

Expression of p53^{Scer\UAS.A.T:Hsap\MYC} under the control of Scer\GAL4^hs.PU does not induce cell death in salivary glands.

(Zhang et al., 2014)

Expression of p53^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PU induces apoptosis in mitotically cycling cells, but not in endocycling cells.

(Mehrotra et al., 2008)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^fkh.PH, Set2^RNAi.UAS, p53^{UAS.A.Tag:MYC} has increased cell death phenotype

(Zhang et al., 2014)

E(Pc)^JF03101, Scer\GAL4^fkh.PH, p53^{UAS.A.Tag:MYC} has increased cell death phenotype

(Zhang et al., 2014)

Nurf-38^GL00369, Scer\GAL4^fkh.PH, p53^{UAS.A.Tag:MYC} has increased cell death phenotype

(Zhang et al., 2014)

G9a^RNAi.UAS.cSa, Scer\GAL4^fkh.PH, p53^{UAS.A.Tag:MYC} has increased cell death phenotype

(Zhang et al., 2014)

Scer\GAL4^fkh.PH, egg^RNAi.UAS.cSa, p53^{UAS.A.Tag:MYC} has increased cell death phenotype

(Zhang et al., 2014)

Scer\GAL4^fkh.PH, Su(var)3-9^HMS00279, p53^{UAS.A.Tag:MYC} has increased cell death phenotype

(Zhang et al., 2014)

Scer\GAL4^fkh.PH, Su(var)3-9^HMS00704, p53^{UAS.A.Tag:MYC} has increased cell death phenotype

(Zhang et al., 2014)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^fkh.PH, Set2^RNAi.UAS, p53^{UAS.A.Tag:MYC} has salivary gland phenotype

(Zhang et al., 2014)

E(Pc)^JF03101, Scer\GAL4^fkh.PH, p53^{UAS.A.Tag:MYC} has salivary gland phenotype

(Zhang et al., 2014)

Nurf-38^GL00369, Scer\GAL4^fkh.PH, p53^{UAS.A.Tag:MYC} has salivary gland phenotype

(Zhang et al., 2014)

G9a^RNAi.UAS.cSa, Scer\GAL4^fkh.PH, p53^{UAS.A.Tag:MYC} has salivary gland phenotype

(Zhang et al., 2014)

Scer\GAL4^fkh.PH, egg^RNAi.UAS.cSa, p53^{UAS.A.Tag:MYC} has salivary gland phenotype

(Zhang et al., 2014)

Scer\GAL4^fkh.PH, Su(var)3-9^HMS00279, p53^{UAS.A.Tag:MYC} has salivary gland phenotype

(Zhang et al., 2014)

Scer\GAL4^fkh.PH, Su(var)3-9^HMS00704, p53^{UAS.A.Tag:MYC} has salivary gland phenotype

(Zhang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

6XMyc-p53A

(Chang et al., 2021)

p53^{Scer\UAS.A.T:Hsap\MYC}

p53^{Scer\UAS.T:Hsap\MYC}

p53^{UAS.A.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections