FlyBase Allele Report: Dmel\α-PheRS[A456G.UAS]

General Information

Symbol

Dmel\α-PheRS^A456G.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0301679

Feature type

allele

Associated gene

Dmel\α-PheRS

Associated Insertion(s)

Carried in Construct

M{UAS-α-PheRS.A456G}

Key Links

Transgenic product class

missense_variant

(Lu et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Lu et al., 2014)

Mutagen

in vitro construct

(Lu et al., 2014)

Progenitor genotype

Carried in construct

M{UAS-α-PheRS.A456G}

Cytology

Description

UAS regulates expression of α-PheRS full-length cDNA, containing an A456G substitution that is predicted to increase the activation error rate of the enzyme.

(Lu et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

α-PheRS

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | progressive, with Scer\GAL4^Act5C.PI

(Lu et al., 2014)

increased cell death, with Scer\GAL4^en-e16E

(Lu et al., 2014)

short lived, with Scer\GAL4^Act5C.PI

(Lu et al., 2014)

Phenotype Manifest In

ommatidium, with Scer\GAL4^ey.PH

(Lu et al., 2014)

wing vein, with Scer\GAL4^en-e16E

(Lu et al., 2014)

Detailed Description

Statement

Reference

Scer\GAL4^ey.PH α-PheRS^{A456G.Scer\UAS} eyes mostly display a regular surface, although a few ommatidia are not properly organized in certain regions. Some individual ones lack photoreceptors, causing ommatidia to be smaller and the retina to display a misaligned pattern.

Animals expressing α-PheRS^{A456G.Scer\UAS} via Scer\GAL4^Act5C.PI show normal climbing ability during the first week, but impaired performance in subsequent weeks. They also have a shortened lifespan compared to controls.

Expression via Scer\GAL4^en-e16E causes wing vein defects in the adult wing and a small increase in apoptotic cells in the wing disc.

(Lu et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^Act5C.PI has increased cell death phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, Df(3R)Exel6198/β-PheRS³²², β-PheRS^A158W, Scer\GAL4^Act5C.PI has increased cell death phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, Df(3R)Exel6198/β-PheRS³²², β-PheRS^A158W, Scer\GAL4^en-e16E has decreased cell number phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, Df(3R)Exel6198/β-PheRS³²², β-PheRS^A158W, Scer\GAL4^en-e16E has abnormal size phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, Df(3R)Exel6198/β-PheRS³²², β-PheRS^A158W, Scer\GAL4^ppl.PP has lethal | nutrition conditional phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, Df(3R)Exel6198/β-PheRS³²², β-PheRS^A158W, Scer\GAL4^Act5C.PI has lethal phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^en-e16E has increased cell death phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^en-e16E has decreased cell number phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^en-e16E has abnormal size phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^ppl.PP has lethal | nutrition conditional phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, Df(3R)Exel6198/β-PheRS³²², β-PheRS^A158W, Scer\GAL4^en-e16E has increased cell death phenotype

(Lu et al., 2014)

Phenotype Manifest In

Other

Statement

Reference

α-PheRS^A456G.UAS, Df(3R)Exel6198/β-PheRS³²², β-PheRS^A158W, Scer\GAL4^ey.PH has ommatidium phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^en-e16E has wing phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^ey.PH has ommatidium phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, Df(3R)Exel6198/β-PheRS³²², β-PheRS^A158W, Scer\GAL4^en-e16E has wing phenotype

(Lu et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

In β-PheRS³²²/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRS^+tLa, Scer\GAL4^ey.PH-mediated expression of α-PheRS^{A456G.Scer\UAS}, results in eyes mostly displaying a regular surface, although a few ommatidia are not properly organized in certain regions. Some individual ones lack photoreceptors, causing ommatidia to be smaller and the retina to display a misaligned pattern.

In β-PheRS³²²/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRS^A158W, Scer\GAL4^ey.PH-mediated expression of α-PheRS^{A456G.Scer\UAS} results in severe disruption of eye organization - most ommatidia are irregular, the entire eye is smaller and the eye surface is rough. The retina and ommatidia lack regular patterns, shapes and organization because of smaller ommatidia and absence of many cells.

Scer\GAL4^Act5C.PI-mediated expression of α-PheRS^{A456G.Scer\UAS} in adults (via use of Scer\GAL80^ts.αTub84B) in a β-PheRS³²²/Df(3R)Exel6198 (β-PheRS null) background rescued with β-PheRS^+tLa results in some elav-positive brain (neuronal) cells entering apoptosis. Many more cells enter apoptosis when this background is rescued with β-PheRS^A158W.

In β-PheRS³²²/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRS^+tLa or β-PheRS^A158W, Scer\GAL4^en-e16E-mediated expression of α-PheRS^{A456G.Scer\UAS} results in decreased wing size , and in some wings entire regions of the posterior compartment are missing. In wing discs, the number of apoptotic cells is increased, while cell size is either similar or slightly bigger than controls, suggesting that cell number (proliferation) must be reduced.

In β-PheRS³²²/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRS^+tLa or β-PheRS^A158W, Scer\GAL4^ppl.PP-mediated expression of α-PheRS^{A456G.Scer\UAS} results in reduced pupariation rates when larvae are raised on Tyrosine-rich (but not alanine- or phenylalanine-rich or standard) food.

(Lu et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99911

w^*; wg^Sp-1/SM1; M{UAS-α-PheRS.A456G}ZH-86Fb

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

α-PheRS^{A456G.Scer\UAS}

α-PheRS^A456G.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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