FlyBase Allele Report: Dmel\mir-210[KO]

General Information

Symbol

Dmel\mir-210^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0301906

Feature type

allele

Associated gene

Dmel\mir-210

Associated Insertion(s)

TI{GAL4}mir-210^KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chen et al., 2014)

Mutagen

ends-out gene targeting

(Chen et al., 2014)

FLPase

(Chen et al., 2014)

I-SceI

(Chen et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{GAL4}mir-210^KO

Cytology

Description

The mir-210 hairpin has been deleted and replaced by sequence encoding the Scer\GAL4 coding region plus a loxP cassette containing the w^+mW.hs marker.

(Chen et al., 2014)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

s-LNv Pdf neuron

(Niu et al., 2019)

Detailed Description

Statement

Reference

mir-210^KO mutant adults have significantly advanced evening peak circadian locomotor activity under light-dark (LD), constant darkness (DD) or DD with 21[o] C:29 [o]C temperature cycles, compared to controls; period and rhythmicity under DD are unaffected, compared to controls; unlike controls, mir-210^KO mutant adults do not have a significant decrease in the arborization of Pdf-positive sLNv dorsal projections between 2 h after lights on and 2 h after lights off under LD conditions.

(Niu et al., 2019)

Under constant darkness, mir-210^KO individuals do not show altered rhythmicity, but show a significantly advanced circadian phase by ~6 h, as compared to controls. Under 12h:12h light:dark cycles, individuals show advanced evening activity onset, while morning anticipation is not affected.

mir-210^KO mutants do not show defects in I-LNv (Pdf-positive) neurons, as compared to controls.

mir-210^KO mutants do not show the daily changes in s-LNv neuron axon termini (i.e. shift from defasciculated state at ZT3 to fasciculated state at ZT15) observed in controls, thus remaining at the fasciculated state.

(Cusumano et al., 2018)

Homozygous males show a significant decrease in median lifespan compared to controls.

3-5 day old homozygotes show reduced climbing ability in a negative geotaxis assay.

(Chen et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

mir-210^KO has abnormal locomotor rhythm | adult stage phenotype, non-enhanceable by Pdf⁰¹

(Niu et al., 2019)

mir-210^KO has abnormal locomotor rhythm | adult stage | recessive phenotype, non-enhanceable by Fas2^ΔmiR-210

(Niu et al., 2019)

NOT suppressed by

Statement

Reference

mir-210^KO has abnormal locomotor rhythm | adult stage phenotype, non-suppressible by Pdf⁰¹

(Niu et al., 2019)

mir-210^KO has abnormal locomotor rhythm | recessive | adult stage phenotype, non-suppressible by Fas2^ΔmiR-210

(Niu et al., 2019)

NOT Enhancer of

Statement

Reference

mir-210^KO is a non-enhancer of abnormal locomotor rhythm | adult stage phenotype of Pdf⁰¹

(Niu et al., 2019)

mir-210^KO is a non-enhancer of abnormal locomotor rhythm | adult stage | recessive phenotype of Fas2^ΔmiR-210

(Niu et al., 2019)

NOT Suppressor of

Statement

Reference

mir-210^KO is a non-suppressor of abnormal locomotor rhythm | recessive | adult stage phenotype of Fas2^ΔmiR-210

(Niu et al., 2019)

mir-210^KO is a non-suppressor of abnormal locomotor rhythm | adult stage phenotype of Pdf⁰¹

(Niu et al., 2019)

Other

Statement

Reference

Fas2[+]/Fas2^ΔmiR-210, mir-210^KO has abnormal locomotor rhythm | dominant | adult stage phenotype

(Niu et al., 2019)

Fas2^UAS.cUa, Scer\GAL4^mir-210-KO, mir-210^KO/mir-210[+] has abnormal locomotor rhythm | adult stage phenotype

(Niu et al., 2019)

Fas2^UAS.cUa, Scer\GAL4^mir-210-KO, Scer\GAL80^Pdf.PS, mir-210^KO/mir-210[+] has abnormal locomotor rhythm | adult stage phenotype

(Niu et al., 2019)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

mir-210^KO/+, Fas2^ΔmiR-210/+ transheterozygous adults have significantly advanced evening peak circadian locomotor activity under light-dark (LD) conditions, compared to controls, unlike either single heterozygote.

Expression of Fas2^UAS.cUa under the control of Scer\GAL4^mir-210-KO in mir-210^KO heterozygous adults results in significantly advanced evening peak circadian locomotor activity under LD conditions, compared to controls; this is still the case when Scer\GAL80^Pdf.PS, but not Scer\GAL80^GMR.PU, is used to restrict expression of Fas2^UAS.cUa.

(Niu et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

mir-210^KO is partially rescued by mir-210^UAS.cUa/Scer\GAL4^mir-210-KO

(Niu et al., 2019)

mir-210^KO is partially rescued by mir-210^UAS.cUa/Scer\GAL4^mir-210-KO/Scer\GAL80^Pdf.PS

(Niu et al., 2019)

mir-210^KO is partially rescued by Scer\GAL4^mir-210-KO/mir-210^UAS.cCa

(Cusumano et al., 2018)

mir-210^KO is partially rescued by Scer\GAL4^mir-210-KO/mir-210^UAS.cCa/Scer\GAL80^Pdf.PS

(Cusumano et al., 2018)

mir-210^KO is partially rescued by Scer\GAL80^cry.PS/Scer\GAL4^mir-210-KO/mir-210^UAS.cCa

(Cusumano et al., 2018)

Comments

Expressing mir-210^UAS.cCa under the control of Scer\GAL4^mir-210-KO (the intrinsic driver of mir-210^KO) mostly rescues the circadian defects of mir-210^KO mutants under either constant darkness or 12h:12h light:dark cycles (LD). although the advanced evening onset under LD is only partially rescued.

Expression under the additional control of Scer\GAL80^cry.PS rescues the advanced evening activity onset but not activity phase delay of mir-210^KO.

Expression under the additional control of Scer\GAL80^Pdf.PS does not rescues the advanced evening activity onset nor the activity phase delay of mir-210^KO mutants.

(Cusumano et al., 2018)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58899

y¹ w^* TI{GAL4}mir-210^KO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

miR-210 KO

(Zhou et al., 2024)

mir-210^KO

(Niu et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close