FB2025_01 , released February 20, 2025
Allele: Dmel\mir-276bKO
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General Information
Symbol
Dmel\mir-276bKO
Species
D. melanogaster
Name
FlyBase ID
FBal0301911
Feature type
allele
Associated gene
Dmel\mir-276b
Associated Insertion(s)
TI{TI}mir-276bKO
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Chen et al., 2014)
Mutagen
ends-out gene targeting
(Chen et al., 2014)
FLPase
(Chen et al., 2014)
I-SceI
(Chen et al., 2014)
cre recombinase
(Chen et al., 2014)
Progenitor genotype
Associated Insertion(s)
TI{TI}mir-276bKO
Cytology
Description

The mir-276b hairpin has been deleted. A single loxP site is present at the site of the deletion.

(Chen et al., 2014)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      mir-276bKO mutants show decreased morning and evening activity peaks, increased daytime and nighttime sleep time, with increased sleep bout number, and decreased circadian rhythmicity; the increased sleep time is observed in rhythmic flies, only.

      (Zhang et al., 2021)

      Homozygous males and females show a significant decrease in median lifespan compared to controls.

      3-5 day old homozygotes show reduced climbing ability in a negative geotaxis assay.

      (Chen et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Statement
      Reference

      mir-276b[+]/mir-276bKO is a non-enhancer of increased sleep phenotype of mir-276aKO

      (Zhang et al., 2021)
      Other
      Statement
      Reference

      mir-276aKO, mir-276b[+]/mir-276bKO has abnormal circadian rhythm | adult stage phenotype

      (Zhang et al., 2021)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      mir-276aKO, mir-276bKO double heterozygotes show decreased circadian rhythmicity, compared to controls.

      (Zhang et al., 2021)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      miR-276bKO
      (Tang et al., 2024, Zhang et al., 2021)
      mir-276bKO
      Name Synonyms
      Secondary FlyBase IDs
        References (5)