FlyBase Allele Report: Dmel\azot[KO]

General Information

Symbol

Dmel\azot^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0302673

Feature type

allele

Associated gene

Dmel\azot

Associated Insertion(s)

TI{TI}azot^KO

Carried in Construct

Genomic Maps

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Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Merino et al., 2015)

Mutagen

ends-out gene targeting

(Merino et al., 2015)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

complex substitution

2R:7,949,343..7,950,504 [+]

Comment:

The azot coding region has been deleted by homologous recombination and replaced with an attP site and P1\loxP-flanked w marker. The boundaries of the deleted region were inferred from primer sequences.

(Merino and Moreno, 2015.4.11)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

The azot coding region has been deleted and replaced with an attP site and a loxP-flanked w[+] marker.

(Merino et al., 2015)

Insertion components

TI{TI}azot^KO

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

decreased cell death

(Merino et al., 2015)

radiation sensitive

(Merino et al., 2015)

short lived

(Merino et al., 2015)

viable

(Merino et al., 2015)

visible

(Merino et al., 2015)

Phenotype Manifest In

wing

(Merino et al., 2015)

Detailed Description

Statement

Reference

These mutants are short lived compared to controls.

(Merino et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

The loss of 'loser' cell elimination seen in azot^KO mutant cells following dm-induced cell competition is rescued upon expression of azot^T:Disc\RFP.

Expression of two copies of azot^T:Disc\RFP rescues the wing defects seen in homozygous azot^KO mutant flies.

Expression of two copies of azot^T:Disc\RFP rescues the increase in vacuoles seen in azot^KO mutant brains.

Expression of two copies of azot^T:Disc\RFP rescues the reduced lifespan seen in azot^KO mutant brains.

(Merino et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

azot^KO

(Merino and Moreno, 2015.4.11)

azot{KO; w+}

(Coelho et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (3)

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