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General Information
Symbol
Dmel\sstndsRNA.shRNA.UASp.2
Species
D. melanogaster
Name
FlyBase ID
FBal0302905
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASp regulatory sequences drive expression of an shRNA targeted against sstn.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Maternal expression of sstndsRNA.shRNA.Scer\UAS.P\T.2 (under the simultaneous control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16) results in defects in early embryos. Pseudocleavage and cellularization furrows extend effectively into the embryo as in controls, but then the membranes undergo abnormal perpendicular expansion from the base of the furrows. The expanded membranes are coated with a cytoskeleton marker (pnut) and are seen to encroach into the space normally occupied by the nuclei, resulting in nuclei with indentations and bottle-like shapes. Maternal expression of sstndsRNA.shRNA.Scer\UAS.P\T.2 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in an abnormal perpendicular membrane expansion the base of cellularization furrows in early embryos.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Maternal heterozygosity for Rho172O substantially suppresses the abnormal perpendicular membrane expansion seen at the base of cellularization furrows in embryos derived from females expressing sstndsRNA.shRNA.Scer\UAS.P\T.2 under the simultaneous control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16. Maternal co-expression of stepScer\UAS.P\T.T:Avic\GFP-EGFP completely suppresses the abnormal perpendicular membrane expansion seen at the base of cellularization furrows in embryos derived from females expressing sstndsRNA.shRNA.Scer\UAS.P\T.2 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
sstndsRNA.shRNA.Scer\UAS.P\T.2
sstndsRNA.shRNA.UASp.2
Name Synonyms
Secondary FlyBase IDs
    References (1)