FB2025_01 , released February 20, 2025
Allele: Dmel\sstnRNAi.UASp.2
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General Information
Symbol
Dmel\sstnRNAi.UASp.2
Species
D. melanogaster
Name
FlyBase ID
FBal0302905
Feature type
allele
Associated gene
Dmel\sstn
Associated Insertion(s)
Carried in Construct
P{UASp-sstn.RNAi.2}
Key Links
Transgenic product class
RNAi_reagent
(Liu et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Liu et al., 2015)
Mutagen
in vitro construct
(Liu et al., 2015)
Progenitor genotype
Carried in construct
P{UASp-sstn.RNAi.2}
Cytology
Description

UASp regulatory sequences drive expression of an shRNA targeted against sstn.

(Liu et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
sstn
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Maternal expression of sstndsRNA.shRNA.Scer\UAS.P\T.2 (under the simultaneous control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16) results in defects in early embryos. Pseudocleavage and cellularization furrows extend effectively into the embryo as in controls, but then the membranes undergo abnormal perpendicular expansion from the base of the furrows. The expanded membranes are coated with a cytoskeleton marker (pnut) and are seen to encroach into the space normally occupied by the nuclei, resulting in nuclei with indentations and bottle-like shapes.

      Maternal expression of sstndsRNA.shRNA.Scer\UAS.P\T.2 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in an abnormal perpendicular membrane expansion the base of cellularization furrows in early embryos.

      (Liu et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Maternal heterozygosity for Rho172O substantially suppresses the abnormal perpendicular membrane expansion seen at the base of cellularization furrows in embryos derived from females expressing sstndsRNA.shRNA.Scer\UAS.P\T.2 under the simultaneous control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16.

      Maternal co-expression of stepScer\UAS.P\T.T:Avic\GFP-EGFP completely suppresses the abnormal perpendicular membrane expansion seen at the base of cellularization furrows in embryos derived from females expressing sstndsRNA.shRNA.Scer\UAS.P\T.2 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16.

      (Liu et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      sstnRNAi.UASp.2
      sstndsRNA.shRNA.Scer\UAS.P\T.2
      sstndsRNA.shRNA.UASp.2
      Name Synonyms
      Secondary FlyBase IDs
        References (2)