FlyBase Allele Report: Hsap\ANKLE2[UAS.Tag:FLAG]

General Information

Symbol

Hsap\ANKLE2^UAS.Tag:FLAG

Species

H. sapiens

Name

FlyBase ID

FBal0302966

Feature type

allele

Associated gene

Hsap\ANKLE2

Associated Insertion(s)

Carried in Construct

PBac{UAS-hANKLE2.FLAG}

Also Known As

P{UASt-hANKLE2}

Key Links

Transgenic product class

cDNA

(Yamamoto et al., 2014)

wild_type

(Yamamoto et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Yamamoto et al., 2014)

wild_type

(Yamamoto et al., 2014)

Mutagen

in vitro construct

(Yamamoto et al., 2014)

Progenitor genotype

Carried in construct

PBac{UAS-hANKLE2.FLAG}

Cytology

Description

UASt regulatory sequences drive expression of a Hsap\ANKLE2 cDNA that is tagged with Tag:FLAG.

(Yamamoto et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\ANKLE2

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates microcephaly

modeled by ZIKV\NS4A^{UAS.Tag:StrepII}

(Shah et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^da.G32/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of decreased size | larval stage phenotype of Ankle2^A

(Thomas et al., 2022)

Scer\GAL4^da.G32/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of abnormal size | third instar larval stage phenotype of Ankle2^A

(Link et al., 2019)

Scer\GAL4^da.G32/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Ankle2^A

(Link et al., 2019)

Scer\GAL4^da.G32/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of lethal - all die before end of pupal stage | temperature conditional phenotype of Ankle2^A

(Link et al., 2019)

Scer\GAL4^insc-Mz1407/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of abnormal size | third instar larval stage phenotype of Ankle2^A

(Link et al., 2019)

Scer\GAL4^insc-Mz1407/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Ankle2^A

(Link et al., 2019)

Scer\GAL4^insc-Mz1407/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of lethal - all die before end of pupal stage | temperature conditional phenotype of Ankle2^A

(Link et al., 2019)

Scer\GAL4^unspecified/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of lethal | recessive phenotype of Ankle2^A

(Yamamoto et al., 2014)

Scer\GAL4^unspecified/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of increased cell death | third instar larval stage phenotype of Ankle2^A

(Yamamoto et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^da.G32/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of larval brain | larval stage phenotype of Ankle2^A

(Thomas et al., 2022)

Scer\GAL4^insc-Mz1407/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of larval brain | third instar larval stage phenotype of Ankle2^A

(Link et al., 2019)

Scer\GAL4^da.G32/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of larval brain | third instar larval stage phenotype of Ankle2^A

(Link et al., 2019)

Scer\GAL4^unspecified/Hsap\ANKLE2^UAS.Tag:FLAG is a suppressor of larval brain | third instar larval stage phenotype of Ankle2^A

(Yamamoto et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Ubiquitous expression of Hsap\ANKLE2^{Scer\UAS.T:Zzzz\FLAG} rescues the recessive lethality caused by Ankle2^A and rescues the brain phenotypes (small size and increased cell death) in third instar Ankle2^A larvae.

(Yamamoto et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

91534

y¹ w^*; PBac{UAS-hANKLE2.FLAG}VK00037

91636

y¹ w^*; PBac{UAS-hANKLE2.FLAG}VK00033

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\ANKLE2^{Scer\UAS.T:Zzzz\FLAG}

Hsap\ANKLE2^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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