FB2025_01 , released February 20, 2025
Allele: Dmel\PPO1A480V
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General Information
Symbol
Dmel\PPO1A480V
Species
D. melanogaster
Name
FlyBase ID
FBal0302995
Feature type
allele
Associated gene
Dmel\PPO1
Associated Insertion(s)
Carried in Construct
PBac{PPO1.A480V}
Key Links
Transgenic product class
missense_variant
(Neyen et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Neyen et al., 2014)
Mutagen
in vitro construct
(Neyen et al., 2014)
Progenitor genotype
Carried in construct
PBac{PPO1.A480V}
Cytology
Description

Genomic fragment from Bac clone CH322-126A13 which contains the PPO1 transcription unit. The amino acid replacement A480V (this is the lesion found in the PPO1Bc mutation) has been introduced into the PPO1 coding sequence.

(Neyen et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
PPO1
Encoded product / tool
PPO1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Larvae carrying PPO1A480V in a PPO1[+] background have melanised crystal cells in circulation, and less frequently and apparently, melanisation is seen in the lymph gland.

      Melanisation at the wound site in response to physical wounding is seen in larvae and adults carrying PPO1A480V in a PPO1[+] background, as occurs in wild type.

      Larvae and adults carrying PPO1A480V in a PPO1Δ background do not show melanisation at the wound site in response to physical wounding, in contrast to wild type.

      (Neyen et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Larvae and adults carrying PPO1A480V in a PPO2Δ background do not show melanisation at the wound site in response to physical wounding, in contrast to wild type.

      (Neyen et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      PPO1A480V
      (Hsi et al., 2023, Neyen et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)