FB2025_01 , released February 20, 2025
Allele: Dmel\CspH45Q.UAS
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General Information
Symbol
Dmel\CspH45Q.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0303661
Feature type
allele
Associated gene
Dmel\Csp
Associated Insertion(s)
Carried in Construct
P{UAS-Csp.H45Q}
Key Links
Transgenic product class
missense_variant
(Bronk et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Bronk et al., 2005)
Mutagen
in vitro construct
(Bronk et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-Csp.H45Q}
Cytology
Description

Amino acid replacement: H45Q.

(Bronk et al., 2005)

UASt regulatory sequences drive expression of mutated Csp coding sequences. The single amino acid mutation is within the 'J-domain'.

(Bronk et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Csp
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of CspH45Q.Scer\UAS in motor neurons under the control of Scer\GAL4D42 does not significantly affect mEJP amplitudes or mEJP frequency.

      Expression of CspH45Q.Scer\UAS in motor neurons under the control of Scer\GAL4D42 increases EJP amplitudes at CspR1 mutant neuromuscular junctions. The mean EJP amplitude in larvae expressing CspH45Q.Scer\UAS is not significantly different to wild-type controls. Although CspH45Q.Scer\UAS expression restores ELP amplitudes to control levels, the CspH45Q.Scer\UAS-mediated effect is not very robust. The coefficient of variation of EJP amplitudes is 0.08 for controls, whereas for CspH45Q.Scer\UAS it's 0.24, indicating expression of CspH45Q.Scer\UAS does not fully restore synaptic transmission.

      (Bronk et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/CspH45Q.UAS rescues CspR1

      (Bronk et al., 2005)
      Partially rescues

      Scer\GAL4Toll-6-D42/CspH45Q.UAS partially rescues CspR1

      (Bronk et al., 2005)
      Comments

      Expression of CspH45Q.Scer\UAS in motor neurons under the control of Scer\GAL4D42 increases EJP amplitudes at CspR1 mutant neuromuscular junctions. The mean EJP amplitude in larvae expressing CspH45Q.Scer\UAS is not significantly different to wild-type controls. Although CspH45Q.Scer\UAS expression restores ELP amplitudes to control levels, the CspH45Q.Scer\UAS-mediated effect is not very robust. The coefficient of variation of EJP amplitudes is 0.08 for controls, whereas for CspH45Q.Scer\UAS it's 0.24, indicating expression of CspH45Q.Scer\UAS does not fully restore synaptic transmission.

      Presynaptic expression of CspH45Q.Scer\UAS, under the control of Scer\GAL4elav-C155 in CspR1 mutants rescues the loss of synaptic boutons, increasing their number to approximately 140% of wild-type levels.

      (Bronk et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CspH45Q.Scer\UAS
      CspH45Q.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)