FlyBase Allele Report: Dmel\Csp[ΔL.UAS]

General Information

Symbol

Dmel\Csp^ΔL.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0303663

Feature type

allele

Associated gene

Dmel\Csp

Associated Insertion(s)

Carried in Construct

P{UAS-Csp.83-113}

Key Links

Transgenic product class

inframe_deletion

(Bronk et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Bronk et al., 2005)

Mutagen

in vitro construct

(Bronk et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Csp.83-113}

Cytology

Description

UASt regulatory sequences drive expression of Csp coding sequences where residues 83-113 are deleted, including the 'L-domain'.

(Bronk et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Csp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Csp^R1), with Scer\GAL4^elav-C155

(Bronk et al., 2005)

abnormal neurophysiology, with Csp^R1, Scer\GAL4^Toll-6-D42

(Bronk et al., 2005)

lethal | temperature conditional, with Csp^R1, Scer\GAL4^Toll-6-D42

(Bronk et al., 2005)

partially lethal | temperature conditional (with Csp^R1), with Scer\GAL4^elav-C155

(Bronk et al., 2005)

viable, with Scer\GAL4^elav-C155

(Bronk et al., 2005)

Phenotype Manifest In

NMJ bouton (with Csp^R1), with Scer\GAL4^elav-C155

(Bronk et al., 2005)

Detailed Description

Statement

Reference

Presynaptic expression of Csp^ΔJ.Scer\UAS under the control of Scer\GAL4^elav-C155 results in approximately 98% viability at 25[o]C.

Motor neuron-specific expression of Csp^ΔL.Scer\UAS (under the control of Scer\GAL4^D42 significantly increases evoked EJP amplitudes at Csp^R1 mutant neuromuscular junctions from 52 to 98% of control EJPs. The increased EJP amplitudes are statistically indistinguishable from wild-type controls. Expression of Csp^ΔL.Scer\UAS does not affect mEJP amplitude or frequency in Csp^R1 mutants.

(Bronk et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Toll-6-D42/Csp^ΔL.UAS rescues Csp^R1

(Bronk et al., 2005)

Scer\GAL4^elav-C155/Csp^ΔL.UAS rescues Csp^R1

(Bronk et al., 2005)

Fails to rescue

Scer\GAL4^Toll-6-D42/Csp^ΔL.UAS fails to rescue Csp^R1

(Bronk et al., 2005)

Scer\GAL4^elav-C155/Csp^ΔL.UAS fails to rescue Csp^R1

(Bronk et al., 2005)

Comments

Expression of Csp^ΔL.Scer\UAS at Csp^R1 mutant neuromuscular junctions (under the control of Scer\GAL4^D42) greatly reduces the abnormally increased frequency of asynchronous release shortly after the evoked EJP.

Expression of Csp^ΔL.Scer\UAS (under the control of Scer\GAL4^D42) restores the thermo-tolerance of evoked release at Csp^R1 mutant neuromuscular junctions. No difference is observed in spontaneous quantal release compared to controls. Neither mEJP frequency nor mEJP amplitude are significantly different between 22[o]C and 32[o]C in Csp^R1 mutants expressing Csp^ΔL.Scer\UAS (under the control of Scer\GAL4^D42).

At 30[o]C, motor neuron-specific expression of Csp^ΔL.Scer\UAS (under the control of Scer\GAL4^D42 partially reverses the abnormally high '[Ca[2+]'] resting levels of Csp^R1 mutant boutons to an intermediate level of 93.4nM. Statistically, '[Ca[2+]'] levels at 30[o]C are significantly different from those seen in Csp^R1 mutants and Csp^R1 mutants expressing Csp^ΔJ.Scer\UAS. At 20[o]C, expression of Csp^ΔL.Scer\UAS has no significant effect on the intraterminal '[Ca[2+]'].

Expression of Csp^ΔL.Scer\UAS in Csp^R1 mutant neuromuscular junctions (under the control of Scer\GAL4^D42) partially restores normal intraterminal

'[Ca[2+]'] levels at 30[o]C. Ca[2+] signals at rest, during and after stimulation, reaches intermediate levels, being significantly higher than those of wild-type controls at 30[o]C, but also clearly lower than those of Csp^R1 mutants.

Expression of Csp^ΔL.Scer\UAS at Csp^R1 mutant neuromuscular junctions (under the control of Scer\GAL4^D42), restores the thermo-tolerance of release: at 32[o]C, the average EJP amplitude is slight reduced by 16% but is not significantly different to wild-type controls. Neither mEJP frequency onor mEJP amplitude is significantly different between the groups at 22 or 32[o]C.

Expression of Csp^ΔL.Scer\UAS at Csp^R1 mutant neuromuscular junctions (under the control of Scer\GAL4^D42) significantly reduces the frequency of asynchronous release shortly after the evoked EJP.

(Bronk et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Csp^ΔL.Scer\UAS

Csp^ΔL.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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