FlyBase Allele Report: Scer\GAL4[DBD.R17D06]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Scer\GAL4^DBD.R17D06

General Information

Symbol

Scer\GAL4^DBD.R17D06

Species

S. cerevisiae

Name

FlyBase ID

FBal0304013

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{R17D06-GAL4.DBD}

Also Known As

R17D06DBD

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Tuthill et al., 2013)

Progenitor genotype

Carried in construct

P{R17D06-GAL4.DBD}

Cytology

Description

The R17D06 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Tuthill et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval mushroom body dopaminergic neuron h1

Comment: when combined with P{R30G08-p65.AD} (combination referred to as 'MB440B')

(Truman et al., 2023)

larval mushroom body dopaminergic neuron k1

Comment: when combined with P{R71D01-p65.AD} (combination referred to as 'SS00616')

(Truman et al., 2023)

lamina intrinsic neuron

Comment: when combined with P{R92A10-p65.AD}

(Tuthill et al., 2013)

transmedullary neuron | subset

Comment: when combined with P{R92A10-p65.AD}

(Tuthill et al., 2013)

Additional Information

Statement

Reference

Marker for

lamina intrinsic neuron

(Tuthill et al., 2013)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Hsap\RELA^AD.R92A10, Ih^HMC03319

(Hu et al., 2015)

abnormal neurophysiology | conditional, with Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R92A10

(Hu et al., 2015)

abnormal optomotor response | conditional, with Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R92A10

(Hu et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\RELA^AD.R92A10, Ih^HMC03319, Scer\GAL4^DBD.R17D06 has abnormal neurophysiology phenotype, enhanceable by Hsap\RELA^AD.R92A10/Scer\GAL4^c202a/Scer\GAL4^21D, Scer\GAL4^DBD.R17D06

(Hu et al., 2015)

Enhancer of

Statement

Reference

Hsap\RELA^AD.R92A10, Scer\GAL4^c202a, Scer\GAL4^21D, Scer\GAL4^DBD.R17D06 is an enhancer of abnormal neurophysiology phenotype of Hsap\RELA^AD.R92A10, Ih^HMC03319, Scer\GAL4^DBD.R17D06

(Hu et al., 2015)

Suppressor of

Statement

Reference

Hsap\RELA^AD.R92A10, Ork1^{Δ-C.UAS.EGFP}, Scer\GAL4^DBD.R17D06 is a suppressor | partially of abnormal neurophysiology phenotype of Ih^f03355

(Hu et al., 2015)

Hsap\RELA^AD.R92A10, VGlut1^JF02689, Scer\GAL4^DBD.R17D06 is a suppressor | partially of abnormal neurophysiology phenotype of Ih^f03355

(Hu et al., 2015)

cac^JF02572, Hsap\RELA^AD.R92A10, Scer\GAL4^DBD.R17D06 is a suppressor | partially of abnormal neurophysiology phenotype of Ih^f03355

(Hu et al., 2015)

Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R92A10, Scer\GAL4^DBD.R17D06 is a suppressor of abnormal neurophysiology phenotype of Ih^f03355

(Hu et al., 2015)

Hsap\RELA^AD.R92A10, Bhal\NaChBac^UAS.cNa, Scer\GAL4^DBD.R17D06 is a suppressor | partially of abnormal neurophysiology phenotype of Ih^f03355

(Hu et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Ih^UAS.K, Hsap\RELA^AD.R92A10, Scer\GAL4^c202a, Scer\GAL4^21D, Scer\GAL4^DBD.R17D06 rescues Ih^f03355

(Hu et al., 2015)

Partially rescues

Ih^UAS.K, Hsap\RELA^AD.R92A10, Scer\GAL4^DBD.R17D06 partially rescues Ih^f03355

(Hu et al., 2015)

Comments

Images (0)

Mutant

Wild-type

Stocks (5)

68951

w¹¹¹⁸; P{R17D06-GAL4.DBD}attP2

86949

w¹¹¹⁸; P{R92A10-p65.AD}attP40; P{R17D06-GAL4.DBD}attP2

89170

w¹¹¹⁸; P{R25A08-p65.AD}attP40; P{R17D06-GAL4.DBD}attP2

603336

w¹¹¹⁸; P{R22E04-p65.AD}attP40/CyO, P{2xTb¹-RFP}CyO; P{R17D06-GAL4.DBD}attP2

603340

w¹¹¹⁸; P{R76F05-p65.AD}attP40/CyO, P{2xTb¹-RFP}CyO; P{R17D06-GAL4.DBD}attP2

Notes on Origin

Discoverer

Comments

Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

(Tuthill et al., 2013)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{DBD.R17D06.T:Zzzz\Zip-}

Scer\GAL4^DBD.R17D06

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections