FlyBase Allele Report: Scer\GAL4[GluRIIB-MI03631-TG4.2]

General Information

Symbol

Scer\GAL4^{GluRIIB-MI03631-TG4.2}

Species

S. cerevisiae

Name

FlyBase ID

FBal0304250

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Mi{Trojan-GAL4.2}GluRIIB^{MI03631-TG4.2}

Carried in Construct

Allele class

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

Mutagen

phiC31 integrase

(Diao et al., 2015)

Progenitor genotype

Mi{MIC}MI03631a

(Diao et al., 2015)

Associated Insertion(s)

Mi{Trojan-GAL4.2}GluRIIB^{MI03631-TG4.2}

Cytology

Description

The attP cassette in the Mi{MIC}GluRIIB^MI03631a insertion has been replaced with a Trojan GAL4 cassette that contains a splice acceptor site followed by the T2A peptide, the Scer\GAL4 coding sequence and an Hsp70 transcription termination signal. The insertion is in the correct orientation to gene trap GluRIIB. The splice acceptor ensures that the T2A-Scer\GAL4 open reading frame is incorporated into the GluRIIB mRNA, while the T2A sequence is expected to truncate the native GluRIIB gene product and promotes the separate translation of the Scer\GAL4 open reading frame. Thus Scer\GAL4 expression is driven by the native GluRIIB regulatory sequences.

(Diao et al., 2015)

Allele components

Component

Use(s)

Inserted element

Mi{Trojan-GAL4.2}

gene trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval hypodermal muscle cell

(Diao et al., 2015)

Additional Information

Statement

Reference

ScerGAL4^{GluRIIB-MI03631-TG4.2} drives expression in larval bodywall muscles.

(Diao et al., 2015)

Marker for

Reflects expression of

GluRIIB

(Bloomington Drosophila Stock Center, 2017, Diao et al., 2015)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval neuromuscular junction | larval stage, with Df(3R)Exel7283/Madm^4S3, Madm^UAS.EGFP, Scer\GAL4^VGlut1-OK371

(Mushtaq et al., 2022)

embryonic/larval neuromuscular junction | larval stage, with Madm^UAS.EGFP

(Mushtaq et al., 2022)

embryonic/larval neuromuscular junction | larval stage, with mTor^UAS.Tag:MYC

(Mushtaq et al., 2022)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Madm^UAS.EGFP, Scer\GAL4^{GluRIIB-MI03631-TG4.2} has abnormal neurophysiology | larval stage phenotype, suppressible by Dysb^e01028/Dysb^e01028

(Mushtaq et al., 2022)

Madm^UAS.EGFP, Scer\GAL4^{GluRIIB-MI03631-TG4.2} has abnormal neurophysiology | larval stage phenotype, suppressible by brp⁶⁹

(Mushtaq et al., 2022)

NOT Enhancer of

Statement

Reference

Thor^HMS01555/Scer\GAL4^{GluRIIB-MI03631-TG4.2} is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

Suppressor of

Statement

Reference

mTor^UAS.Tag:MYC/Scer\GAL4^{GluRIIB-MI03631-TG4.2} is a suppressor of abnormal neurophysiology | larval stage phenotype of Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

mTor^UAS.Tag:MYC/Scer\GAL4^{GluRIIB-MI03631-TG4.2} is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

NOT Suppressor of

Statement

Reference

Thor^HMS01555/Scer\GAL4^{GluRIIB-MI03631-TG4.2} is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

Madm^UAS.EGFP, Scer\GAL4^{GluRIIB-MI03631-TG4.2} has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by brp⁶⁹

(Mushtaq et al., 2022)

Madm^UAS.EGFP, Scer\GAL4^{GluRIIB-MI03631-TG4.2} has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by Dysb^e01028/Dysb^e01028

(Mushtaq et al., 2022)

NOT Enhancer of

Statement

Reference

mTor^UAS.Tag:MYC/Scer\GAL4^{GluRIIB-MI03631-TG4.2} is a non-enhancer of NMJ bouton | larval stage | decreased number phenotype of Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

Thor^HMS01555/Scer\GAL4^{GluRIIB-MI03631-TG4.2} is a non-enhancer of NMJ bouton | larval stage | decreased number phenotype of Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

Suppressor of

Statement

Reference

mTor^UAS.Tag:MYC/Scer\GAL4^{GluRIIB-MI03631-TG4.2} is a suppressor | partially of synapse | larval stage phenotype of Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

mTor^UAS.Tag:MYC/Scer\GAL4^{GluRIIB-MI03631-TG4.2} is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

NOT Suppressor of

Statement

Reference

Thor^HMS01555/Scer\GAL4^{GluRIIB-MI03631-TG4.2} is a non-suppressor of NMJ bouton | larval stage | decreased number phenotype of Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

mTor^UAS.Tag:MYC/Scer\GAL4^{GluRIIB-MI03631-TG4.2} is a non-suppressor of NMJ bouton | larval stage | decreased number phenotype of Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155, Scer\GAL4^{GluRIIB-MI03631-TG4.2}, Madm^UAS.EGFP rescues Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

Partially rescues

Scer\GAL4^{GluRIIB-MI03631-TG4.2}/Madm^UAS.EGFP partially rescues Df(3R)Exel7283/Madm^4S3

(Mushtaq et al., 2022)

Fails to rescue

Scer\GAL4^{GluRIIB-MI03631-TG4.2}/Madm^UAS.EGFP fails to rescue Df(3R)Exel7283/Madm^2D2

(Mushtaq et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

60333

y¹ w^*; Mi{Trojan-GAL4.2}GluRIIB^{MI03631-TG4.2}; Dr¹/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^{GluRIIB-MI03631-TG4.2}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections