FlyBase Allele Report: Scer\GAL4[VGlut1-MI04979-TG4.2]

General Information

Symbol

Scer\GAL4^{VGlut1-MI04979-TG4.2}

Species

S. cerevisiae

Name

FlyBase ID

FBal0304251

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Mi{Trojan-GAL4.2}VGlut1^{MI04979-TG4.2}

Carried in Construct

Also Known As

vGlut-GAL4, VGlut^MI04979-Gal4

Allele class

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

Mutagen

phiC31 integrase

(Diao et al., 2015)

Progenitor genotype

Mi{MIC}VGlut1^MI04979

(Diao et al., 2015)

Associated Insertion(s)

Mi{Trojan-GAL4.2}VGlut1^{MI04979-TG4.2}

Cytology

Description

The attP cassette in the Mi{MIC}VGlut^MI04979 insertion (which is in a coding intron common to all splice forms of VGlut) has been replaced with a Trojan GAL4 cassette that contains a splice acceptor site followed by the T2A peptide, the Scer\GAL4 coding sequence and an Hsp70 transcription termination signal. The insertion is in the correct orientation to gene trap VGlut. The splice acceptor ensures that the T2A-Scer\GAL4 open reading frame is incorporated into the VGlut mRNA, while the T2A sequence is expected to truncate the native VGlut gene product and promotes the separate translation of the Scer\GAL4 open reading frame. Thus Scer\GAL4 expression is driven by the native VGlut regulatory sequences.

(Diao et al., 2015)

Allele components

Component

Use(s)

Inserted element

Mi{Trojan-GAL4.2}

gene trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

glutamatergic neuron

(Diao et al., 2015)

embryonic/larval motor neuron

(Diao et al., 2015)

larval DA1 motor neuron

(Diao et al., 2015)

larval RP2 motor neuron

(Diao et al., 2015)

adult stage

DN1 neuron | subset

(Guo et al., 2016)

glutamatergic neuron

(Hill et al., 2019, Lacin et al., 2019)

medullary intrinsic neuron Mi9

(Davis et al., 2020)

adult glutamatergic neuron

(McKinney et al., 2020)

adult octopaminergic neuron | subset

(McKinney et al., 2020)

Additional Information

Statement

Reference

ScerGAL4^{VGlut-MI04979-TG4.2} drives expression in neurons in the adult thoracico-abdominal ganglion that express VGlut1 but not ChAT or GABA.

(Lacin et al., 2019)

Drives expression in glutamatergic neurons.

(FlyBase Curators, 2017)

ScerGAL4^{VGlut-MI04979-TG4.2} drives expression in adult neurons that use glutamate as a neurotransmitter, including a subset of DN1 neurons in the adult dorsal brain that coexpress EcollexA^GMR18H11.

(Guo et al., 2016)

ScerGAL4^{VGlut-MI04979-TG4.2} drives expression in neurons that use glutamate as a neurotransmitter, and replicates the VGlut1 immunoreactivity pattern. Expression is also observed in a small group of neurons in the posterior larval optic lobe; those neurons are not positive for VGlut1 immunoreactivity, but are also labeled by the VGlut1 reporter ScerGAL4^VGlut-OK371

(Diao et al., 2015)

Marker for

adult glutamatergic neuron

(McKinney et al., 2020)

Reflects expression of

VGlut1

(McKinney et al., 2020, Hill et al., 2019, Lacin et al., 2019, Bloomington Drosophila Stock Center, 2017, Guo et al., 2016, Diao et al., 2015)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response | adult stage, with sei^JF01474

(Hill et al., 2019)

abnormal locomotor behavior | larval stage | conditional, with Crei\ChR2^UAS.cSa

(Riabinina et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Crei\ChR2^UAS.cSa with 470 nm (blue) light) of Scer\GAL4^{VGlut-MI04979-TG4.2} cells results in loss of larval mobility.

(Riabinina et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

60312

w^*; Mi{Trojan-GAL4.2}VGlut1^{MI04979-TG4.2}/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Scer\GAL4^{VGlut-MI04979-TG4.2}

Scer\GAL4^{VGlut1-MI04979-TG4.2}

VGlut^{MI04979-TG4DBD}

(Yoshikawa et al., 2024)

VGlut^MI04979

(Davla et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

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