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General Information
Symbol
Dmel\Gr5aLEXA
Species
D. melanogaster
Name
FlyBase ID
FBal0304286
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The Gr5a coding sequence has been replaced with sequence encoding a lexA::VP16 driver.

The Gr5a coding sequence has been replaced with sequence encoding a lexA::VP16 driver. A single loxP site is also present downstream of the inserted driver sequence.

Insertion components
TI{lexA::VP16}Gr5aLEXA
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous Gr5aLEXA mutant flies exhibit a reduced proboscis extension response to maltose and trehalose upon stimulation of either the labial or tarsal taste sensilla, and several other sugars upon stimulation of the tarsal sensilla.

Gr61a1 Gr5aLEXA Df(3L)ΔGr64 mutants expressing P{t15.3.R1} and P{UAS-Rescue.R2} (i.e. lacking all 8 sweet gustatory receptor genes) do not exhibit any defects in fructose preference in two-choice preference assays.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Adult females homozygous for Gr5aLEXA, Gr61a1 and Df(3L)ΔGr64, or with Gr5aLEXA/+, Gr61a1/+ and Gr64fLEXA/Df(3L)ΔGr64, have a significant increase in preference for softer 100 mM sucrose-containing, but not plain, agarose for egg laying, compared to controls; these mutants also have a significant reduction in preference for 100 mM sucrose-containing (rather than plain) agarose for egg laying, compared to controls.

Adults with homozygous Gr5aLEXA, Gr61a1 and Df(3L)ΔGr64 have proboscis extension rates that are significantly reduced in response to sucrose and significantly increased in response to some fatty acids, compared to controls.

Gr5aLEXA, Gr61a1, Df(3L)ΔGr64 triple homozygotes that also bear P{t15.3.R1} (P{t15.3.R1} rescues the lethality of Df(3L)ΔGr64 homozygosity) have impaired responsiveness to sweet chemical: sweet taste 5b sensilla GRNs virtually lack the expected responsiveness to the chemicals Sucrose, Fructose, Maltose, Trehalose, Glucose, Melezitose, Arabinose and Glycerol, as compared to controls; sweet taste 5v sensilla GRNs show the expected responsive only to Sucrose and Fructose, but lack responses to Maltose, Trehalose and Glucose.

Expressing Gr64aUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores the response of 5v sensilla GRNs only to Maltose but does not affect the responses of 5b sensilla GRNs.

Expressing Gr61aUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores a moderate response of 5v sensilla GRNs only to Glucose but does not affect the responses of 5b sensilla GRNs.

Expressing Gr5aUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores a small response of 5v sensilla GRNs only to Trehalose but does not affect the responses of 5b sensilla GRNs.

Co-expressing Gr64aUAS.cUa and Gr64eUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores the response of 5b sensilla GRNs only to Maltose and Sucrose, but does not affect the responses by 5v sensilla GRNs.

Co-expressing Gr64bUAS.cUa and Gr64eUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores the response of 5b sensilla GRNs only to Glycerol, but does not affect the responses by 5v sensilla GRNs.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)