FlyBase Allele Report: Dmel\Nhe2[null]

General Information

Symbol

Dmel\Nhe2^null

Species

D. melanogaster

Name

FlyBase ID

FBal0304403

Feature type

allele

Associated gene

Dmel\Nhe2

Associated Insertion(s)

Carried in Construct

Also Known As

DNhe2^null

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Grillo-Hill et al., 2015)

Progenitor genotype

PBac{WH}f02217

(Grillo-Hill et al., 2015)

P{XP}Nhe2^d05535

(Grillo-Hill et al., 2015)

Caused by aberration

Df(2L)Nhe2^null

Cytology

Description

All Nhe2^null exons have been excised.

(Grillo-Hill et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:21,107,732..21,135,979 [+]

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them. All exons of Nhe2 have been removed.

(Grillo-Hill et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | oogenesis

(Ulmschneider et al., 2016)

decreased cell number | oogenesis

(Ulmschneider et al., 2016)

decreased fecundity

(Ulmschneider et al., 2016)

partially lethal - majority die

(Grillo-Hill et al., 2015)

Phenotype Manifest In

basal stalk

(Ulmschneider et al., 2016)

female germline cyst

(Ulmschneider et al., 2016)

female germline stem cell | oogenesis

(Ulmschneider et al., 2016)

follicle cell

(Ulmschneider et al., 2016)

germarium

(Ulmschneider et al., 2016)

retina

(Grillo-Hill et al., 2015)

secondary pigment cell

(Grillo-Hill et al., 2015)

stalk follicle cell

(Ulmschneider et al., 2016)

Detailed Description

Statement

Reference

Nhe2^null homozygous females lay significantly fewer eggs compared to controls. Their germaria show frequent defects including cyst encapsulation defects and/or disorganized follicular epithelium, and loss of germline stem cells, as compared to controls. When stalks were present in the ovarioles, stalk cells exhibited signs of differentiation defects, illustrated by ectopic expression of Eya and a mostly rounded morphology, as compared to controls.

(Ulmschneider et al., 2016)

Retinal architecture in homozygous Nhe2^null mutant flies is organized and regular, resembling wild type. In third instar larval eye discs tissue morphology is indistinguishable from wild type but there are fewer proliferative cells than in wild type. In pupal eyes, the secondary pigment cells are thicker than in wild type and cell shapes and cell contacts are irregular.

The intracellular pH (pHi) in Nhe2^null eye discs is significantly lower than in controls, but is unchanged from wild type by the pupal stages.

(Grillo-Hill et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Nhe2^null, Raf^UAS.F179, Scer\GAL4^GMR.PU has cell lethal phenotype

(Grillo-Hill et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Nhe2^null, Scer\GAL4^GMR.PU is an enhancer of retina phenotype of Raf^UAS.F179, Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

Nhe2^null, Scer\GAL4^GMR.PU is an enhancer of rhabdomere phenotype of Raf^UAS.F179, Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

Suppressor of

Statement

Reference

Nhe2[+]/Nhe2^null is a suppressor of ovary phenotype of Ae2¹

(Benitez et al., 2019)

Nhe2[+]/Nhe2^null is a suppressor of ovariole phenotype of Ae2¹

(Benitez et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of phl^{Scer\UAS.F179} under the control of Scer\GAL4^GMR.PU in a Nhe2^null mutant background results in synthetic cell lethality. Externally, the adult eye phenotype is identical to expression of phl^{Scer\UAS.F179} alone, except that eye colour is lighter, but internally there is loss of tissue architecture and cell structure with coagulative necrosis, including loss of rhabdomere morphology, absence of distinct cell membranes and diffusion of pigment granules.

Nhe2^null suppresses the tissue morphology defects and hyperproliferation seen in third instar larval eye disc when phl^{Scer\UAS.F179} is expressed under the control of Scer\GAL4^GMR.PU. Neuronal cell fates appear unaffected.

Nhe2^null enhances the morphological defects seen in the retina of pupal expressing phl^{Scer\UAS.F179} under the control of Scer\GAL4^GMR.PU. All cell types appear smaller and more cells have abnormal morphologies. More ommatidia have extra cone cells. Cell death is seen that is not detected in either mutant alone.

In contrast to either mutant alone, the intracellular pH (pHi) in eye discs expressing phl^{Scer\UAS.F179} under the control of Scer\GAL4^GMR.PU in a Nhe2^null mutant background is similar to wild type. However, the pHi is reduced compared to wild type by the pupal stages.

(Grillo-Hill et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nhe2^null is rescued by Scer\GAL4^Mef2.PR/Nhe2^UAS.cGa

(Grillo-Hill et al., 2015)

Not rescued by

Nhe2^null is not rescued by Scer\GAL4^elav.PU/Nhe2^UAS.cGa

(Grillo-Hill et al., 2015)

Comments

The semi-lethality of Nhe2^null flies is rescued by expression of Nhe2^Scer\UAS.cGa using the muscle-specific Scer\GAL4^Mef2.PR driver, but not using the neuronal Scer\GAL4^elav.PU driver.

(Grillo-Hill et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DNhe2^null

(Ulmschneider et al., 2016)

Nhe2^null

dNhe2^null

(Benitez et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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