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General Information
Symbol
Zzzz\GGGGCC36.UAS
Species
a. artificial
Name
FlyBase ID
FBal0304408
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of 36 pure repeats of Zzzz\GGGGCC.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Zzzz\GGGGCC36.Scer\UAS leads to a rough eye phenotype (Scer\GAL4GMR.PF-driven expression) and to significant decreases in both larval locomotor activity in crawling assays (Scer\GAL4nSyb.PS-driven expression) and adult locomotor activity in climbing assays (Scer\GAL4da.G32-driven expression), as compared to controls.

The expression of Zzzz\GGGGCC36.UAS under the control of Scer\GAL4CCAP.PI leads a significant decrease in the axonal transport of both mitochondria (i.e. a significant decrease in retrograde transport and a significant increase in the stationary fraction) and (NPY-positive) vesicles (i.e. a significant decrease in anterograde transport and a significant increase in the stationary fraction), in third instar larval motor neurons, as compared to controls.

Expression under the control of Scer\GAL4Toll-6-D42 does not lead to any changes in larval or adult locomotion (in climbing and crawling assays, respectively), as compared to controls.

Expression of Zzzz\GGGGCC36.Scer\UAS under the control of Scer\GAL4GMR.PU results in eye-degenerative phenotype.

Constitutive expression of Zzzz\GGGGCC36.Scer\UAS in the eye using Scer\GAL4GMR.PU causes eye degeneration. Increasing expression levels of the pure repeats by increasing the temperature leads to lethality.

Adult-onset expression of Zzzz\GGGGCC36.Scer\UAS under the control of Scer\GAL4elav.Switch.PO (in combination with RU486 treatment) kills all flies by 30 days after eclosion.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Phenotype Manifest In
Suppressed by
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The co-expression of SF2GD12097, but not of Ref1KK109076 or Ref1GD4683, fully suppresses the rough eye, fully suppresses the decreased adult locomotor activity, and partially suppresses the decreased larval locomotor activity induced by the expression of Zzzz\GGGGCC36.Scer\UAS under the control of Scer\GAL4GMR.PF, Scer\GAL4da.G32, and Scer\GAL4nSyb.PS, respectively.

The decreases in both larval and adult locomotor activity induced by the expression of Zzzz\GGGGCC36.Scer\UAS under the control of Scer\GAL4nSyb.PS and Scer\GAL4da.G32, respectively, are suppressed by the co-expression of sbrHM05135.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Zzzz\GGGGCC36.Scer\UAS
Zzzz\GGGGCC36.UAS
Zzzz\poly-GP36.Scer\UAS
Zzzz\poly-GR36.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
  • FBal0300893
  • FBal0300901
References (9)