Inhibition (using Hsap\KCNJ2^UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, does not affect food intake or climbing rate, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01 in adults does not affect food intake or climbing rate, compared to controls.

Inhibition of synaptic release (using shi^{ts1.IVS.p10.20xUAS} at 32[o]C) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01 during training and testing significantly impairs aversive odor-associated short term memory in adults, compared to controls; these flies also have significantly impaired aversive odor-associated short term memory when these neurons are inhibited (at 27[o]C) during testing only.

Inhibition (using shi^{ts1.UAS.Tag:MYC} at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01 in adults during training, consolidation or retrieval significantly reduces 2 h appetitive, but not aversive, olfactory memory, compared to controls.

Inhibition of the combined set of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.Mef2.247 and Hsap\RELA^AD.12xCRE and neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01 in adults during training, consolidation or retrieval, significantly reduces 2 h appetitive, but not aversive, olfactory memory, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R70B10, but not Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01, or Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R20G03, in adult males significantly increases the amount of sleep, compared to controls; activation of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R70B10, but not Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01, or Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R20G03, significantly increases locomotor activity of awake adult males, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31.5[o]C) of neurons targeted by the split-GAL4 pairs of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01, or Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R73H08, in non-sleep deprived adult males does not affect the amount of sleep, compared to controls; inhibition after sleep deprivation in adult males or females significantly reduces the amount of extra rebound sleep, compared to controls; inhibition does not significantly affect locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R73H08 in adult females in an area of an arena significantly increases attraction to that area, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R73H08 in adults significantly increases sleep, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01 in adult females in an area of an arena significantly increases attraction to that area, compared to controls; simultaneous activation of these cells and neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R13F04 and Hsap\RELA^AD.R93D10 also increases attraction, to a greater extent than either subset alone; there is no effect on angular speed or walking speed, compared to baseline, similar to controls, in either of these combinations; simultaneous activation of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01 and neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R52G04, or the split-GAL4 pair of Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R71D08, also increases attraction, to a greater extent, or a similar extent, respectively, compared to either subset alone.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01 in adults during training, consolidation and testing does not substantially reduce aversive 2 h associative olfactory memory, but this (or inhibition using shi^1.UAS) does reduce attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction. Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) does not significantly affect aversive or appetitive 1 min associative visual memory, compared to controls; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls; inhibition only during training and testing significantly reduces 24 h appetitive, but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa or TrpA1^{20xUAS.JFRC124} at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01 in adults significantly increases sleep, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R20G03 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS}, but not shi^1.UAS, at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R20G03 in adults during training, consolidation and testing substantially reduces aversive 2 h associative olfactory memory, compared to controls; inhibition (using shi^{ts1.IVS.p10.20xUAS} or shi^1.UAS, at 32[o]C) substantially reduces attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction. Inhibition of these neurons (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) during training, consolidation and testing does not significantly affect appetitive 1 min associative visual memory, compared to controls; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls; inhibition during training and testing significantly reduces 24 h appetitive (to the extent that this is aversive), but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R20G03 in adults does not significantly affect sleep, compared to controls, and there is no effect on social or locomotor behaviors.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R70B10 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R70B10 in adults during training, consolidation and testing does not substantially reduce aversive 2 h associative olfactory memory, or aversive or appetitive 1 min associative visual memory, compared to controls; inhibition (using shi^{ts1.IVS.p10.20xUAS} or shi^1.UAS, at 32[o]C) substantially reduces attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction; inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls nad inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol.

Thermogenetic activation (using TrpA1^UAS.(B).cKa or TrpA1^{20xUAS.JFRC124} at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R70B10 in adults significantly increases sleep, compared to controls, with no effect on social or locomotor behaviors.