Inhibition (using Hsap\KCNJ2^UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C06 and Hsap\RELA^AD.R40B08, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, does not affect food intake or climbing rate, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C06 and Hsap\RELA^AD.R40B08 in adults does not affect food intake or climbing rate, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C06 and Hsap\RELA^AD.R40B08 in adults during retrieval alone, but not acquisition and consolidation, significantly reduces 24-hour associative olfactory water-reward memory, compared to controls.

Blocking protein synthesis (using Rcom\RA[cs2.UAS] at 30[o]C) for the full 24 h, or the first 12 h, but not the first 8 or 10 h, nor other 12 h intervals, between spaced training and testing in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C06 and Hsap\RELA^AD.R40B08, significantly impairs aversive olfactory long term memory in adults, compared to controls; there is no effect when protein synthesis is instead blocked for 24 h prior to training.

Acute inhibition of endocytosis (using shi^1.UAS at 32[o]C) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C06 and Hsap\RELA^AD.R40B08 during testing, 24 h after spaced, but not massed, training significantly impairs aversive olfactory long term memory in adults, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C06 and Hsap\RELA^AD.R40B08 in adult males does not significantly affect the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C06 and Hsap\RELA^AD.R40B08 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C06 and Hsap\RELA^AD.R40B08 in adults during training, consolidation and testing does not substantially reduce aversive 2 h associative olfactory memory, compared to controls; inhibition (using shi^{ts1.IVS.p10.20xUAS} or shi^1.UAS, at 32[o]C) substantially reduces attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction. Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) does not significantly affect aversive or appetitive 1 min associative visual memory, compared to controls; inhibition during testing alone significantly reduces aversive 2 h associative olfactory memory, and inhibition only during training and testing significantly reduces 24 h appetitive, but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C06 and Hsap\RELA^AD.R40B08 in adults does not significantly affect sleep, compared to controls, and there is no effect on social or locomotor behaviors.