FlyBase Allele Report: Scer\GAL4[DBD.R39A11]

General Information

Symbol

Scer\GAL4^DBD.R39A11

Species

S. cerevisiae

Name

FlyBase ID

FBal0304456

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R39A11-GAL4.DBD}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{R39A11-GAL4.DBD}

Cytology

Description

The R39A11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR39A11

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

gamma dorsal Kenyon cell

Comment: when combined with P{R19B03-p65.AD} (combination referred to as 'MB607B')

when combined with P{R26E07-p65.AD} (combination referred to as 'MB419B')

(Yamazaki et al., 2018, Vogt et al., 2016, Aso et al., 2014)

adult brain | restricted

Comment: when combined with P{R25F11-p65.AD}

when combined with P{R60E02-p65.AD}

(Hampel et al., 2015)

adult ventral nerve cord | restricted

Comment: when combined with P{R25F11-p65.AD}

when combined with P{R60E02-p65.AD}

(Hampel et al., 2015)

zone C/E Johnston organ neuron

Comment: when combined with P{R25F11-p65.AD}

when combined with P{R60E02-p65.AD}

(Hampel et al., 2015)

gamma dorsal Kenyon cell | intense

Comment: when combined with P{R19B03-p65.AD} (combination referred to as 'MB607B')

(Aso et al., 2014)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | conditional, with Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R19B03

(Zhao et al., 2018)

abnormal grooming behavior | heat sensitive, with Hsap\RELA^AD.R25F11, TrpA1^UAS.(B).cKa

(Hampel et al., 2015)

abnormal grooming behavior | heat sensitive, with Hsap\RELA^AD.R60E02, TrpA1^UAS.(B).cKa

(Hampel et al., 2015)

abnormal locomotor behavior | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, TrpA1^UAS.(B).cKa

(Sitaraman et al., 2015)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R19B03, TrpA1^UAS.(B).cKa

(Yamazaki et al., 2018)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, shi^{ts1.IVS.p10.20xUAS}

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, shi^{ts1.UAS.Tag:MYC}

(Yamazaki et al., 2018)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, TrpA1^UAS.(B).cKa

(Yamazaki et al., 2018)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R26E07, shi^{ts1.IVS.p10.20xUAS}

(Vogt et al., 2016)

abnormal sleep | adult stage | female limited | heat sensitive, with Hsap\RELA^AD.R26E07, TrpA1^UAS.(B).cKa

(Sitaraman et al., 2015)

abnormal sleep | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, shi^{ts1.IVS.p10.20xUAS}

(Sitaraman et al., 2015)

abnormal sleep | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, TrpA1^UAS.(B).cKa

(Sitaraman et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using shi^{ts1.UAS.Tag:MYC} at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R19B03 in adults during retrieval, but not training or consolidation, significantly reduces 2 h aversive olfactory memory, compared to controls; inhibition during training, consolidation or retrieval significantly reduces 2 h appetitive olfactory memory, compared to controls; inhibition in combination with a paired odor results in a significant increase in short-term associative aversive olfactory memory in fed or starved flies, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R19B03 in adults during training, consolidation or retrieval significantly reduces 2 h aversive, but not appetitive, olfactory memory, compared to controls; thermogenetic activation in combination with a paired odor, does not affect short-term associative olfactory memory in fed or starved flies, compared to controls.

(Yamazaki et al., 2018)

Adult males with inhibition (using Ctet\tetX^TNT-LC.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R19B03 have significantly weaker suppression of courtship behavior following training with mated females, compared to controls.

(Zhao et al., 2018)

Blocking synaptic transmission (using shi^{ts1.IVS.p10.20xScer\UAS} at 33[o]C) from neurons targeted by the split-GAL4 combination of Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R19B03 or Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R26E07 significantly impairs visual (aversive color and brightness discrimination conditioning) but not olfactory (aversive conditioning) memory performance, compared to controls.

(Vogt et al., 2016)

Thermogenetic activation (using TrpA1^{Scer\UAS.(B).cKa} at 30-31[o]C) of neurons targeted by the following split-GAL4 pairs results in a significant increase of antennal grooming behavior, compared to controls: Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R60E02, Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R25F11.

(Hampel et al., 2015)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R19B03 in adult males or females significantly increases the amount of sleep, compared to controls; activation of neurons targeted by Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R26E07 significantly increases the amount of sleep in adult females, but not males, compared to controls; in the case of Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R19B03, but not Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R26E07, activation significantly increases locomotor activity of awake adult males, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R19B03 after sleep deprivation in adult males significantly reduces the amount of extra rebound sleep, compared to controls.

(Sitaraman et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

69022

w¹¹¹⁸; P{R39A11-GAL4.DBD}attP2

601562

w¹¹¹⁸; P{R39A11-GAL4.DBD}attP40

68256

w¹¹¹⁸; P{R19B03-p65.AD}attP40; P{R39A11-GAL4.DBD}attP2

68323

w¹¹¹⁸; P{R26E07-p65.AD}attP40/CyO; P{R39A11-GAL4.DBD}attP2

Notes on Origin

Discoverer

Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

(Aso et al., 2014)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{DBD.R39A11.T:Zzzz\Zip-}

Scer\GAL4^DBD.R39A11

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

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