FB2025_05 , released December 11, 2025
Allele: Scer\GAL4DBD.R48B03
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General Information
Symbol
Scer\GAL4DBD.R48B03
Species
S. cerevisiae
Name
FlyBase ID
FBal0304461
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{R48B03-GAL4.DBD}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Aso et al., 2014)
Progenitor genotype
Carried in construct
P{R48B03-GAL4.DBD}
Cytology
Description

The R48B03 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
GMR48B03
Encoded product / tool
GAL4(DBD)::Zip-
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      dopaminergic PAM neuron 12

      Comment: when combined with P{R30G08-p65.AD} (combination referred to as 'MB441B')

      (Aso et al., 2014)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Acute thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R48B03 and Hsap\RELAAD.R30G08, in combination with a paired odor, in adults results in a significant increase in short-term associative aversive olfactory memory, compared to controls.

      Inhibition (using shits.UAS.cUa at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R48B03 and Hsap\RELAAD.R30G08 in adults significantly reduces short-term associative appetitive, but not aversive, olfactory memory, compared to controls, with no effect on sugar preference; 30-min appetitive olfactory memory is also reduced when inhibition occurs only during conditioning, compared to controls.

      Acute inhibition (using shits.UAS.cUa at 30[o]C or Npha\HReNpHR3.UAS.YFP with 591 nm (yellow) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R48B03 and Hsap\RELAAD.R30G08, in combination with a paired odor, in adults results in a significant increase in short-term associative attractive olfactory memory, compared to controls.

      Blocking Gαo signalling (using Bper\ptxAact.UAS) in neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R48B03 and Hsap\RELAAD.R30G08 in adults significantly reduces short-term associative appetitive olfactory memory, compared to controls, with no effect on sugar preference.

      (Yamagata et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Aso et al., 2014)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.R48B03.T:Zzzz\Zip-
      Scer\GAL4DBD.R48B03
      Name Synonyms
      Secondary FlyBase IDs
        References (22)