FlyBase Allele Report: Scer\GAL4[DBD.ple]

General Information

Symbol

Scer\GAL4^DBD.ple

Species

S. cerevisiae

Name

FlyBase ID

FBal0304489

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{ple-GAL4.DBD}

PBac{ple-GAL4.DBD}

Also Known As

TH-ZpGAL4DBD, TH-DBD

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{ple-GAL4.DBD}

PBac{ple-GAL4.DBD}

Cytology

Description

The ple promoter region is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

dopaminergic PAM neuron 5 | faint

Comment: when combined with P{R30G08-p65.AD} (combination referred to as 'MB032B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 6

Comment: when combined with P{R30G08-p65.AD} (combination referred to as 'MB032B')

(Dolan et al., 2018, Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 12 | faint

Comment: when combined with P{R30G08-p65.AD} (combination referred to as 'MB032B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 3 | faint

Comment: when combined with P{R30G08-p65.AD} (combination referred to as 'MB032B')

(Yamagata et al., 2015, Aso et al., 2014)

MB-alpha neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{R52H03-p65.AD} (combination referred to as 'MB504B')

(Vogt et al., 2014)

mushroom body medial-vertical lobe arborizing neuron 1

Comment: when combined with P{R52H03-p65.AD} (combination referred to as 'MB504B')

(Aso et al., 2014, Vogt et al., 2014)

mushroom body pedunculus-medial lobe arborizing neuron 1

Comment: when combined with P{R52H03-p65.AD} (combination referred to as 'MB504B')

(Aso et al., 2014, Vogt et al., 2014)

mushroom body vertical lobe arborizing neuron 1

Comment: when combined with P{R52H03-p65.AD} (combination referred to as 'MB504B')

(Aso et al., 2014, Vogt et al., 2014)

adult dopaminergic PAM neuron | subset

Comment: when combined with P{R24E12-p65.AD}

when combined with P{R58E02-p65.AD}

when combined with P{R76F05-p65.AD}

(Cichewicz et al., 2017)

dopaminergic PPL1 neuron | subset

Comment: when combined with P{R52H03-p65.AD}

when combined with P{R76F05-p65.AD}

when combined with P{R82C10-p65.AD}

(Cichewicz et al., 2017)

dopaminergic PPM3 neuron | subset

Comment: when combined with P{R52H03-p65.AD}

when combined with P{R76F05-p65.AD}

(Cichewicz et al., 2017)

dopaminergic PAL neuron | subset

Comment: when combined with P{R76F05-p65.AD}

(Cichewicz et al., 2017)

dopaminergic PPM1 neuron | subset

Comment: when combined with P{R76F05-p65.AD}

(Cichewicz et al., 2017)

dopaminergic PPM2 neuron | subset

Comment: when combined with P{R76F05-p65.AD}

(Cichewicz et al., 2017)

dopaminergic PPL2ab neuron | subset

Comment: when combined with P{R76F05-p65.AD}

(Cichewicz et al., 2017)

dopaminergic PAM neuron 1 | faint

Comment: when combined with P{R30G08-p65.AD} (combination referred to as 'MB032B')

(Aso et al., 2014)

MB-alpha neuron of the dopaminergic PPL1 cluster | faint

Comment: when combined with P{R52H03-p65.AD} (combination referred to as 'MB504B')

(Aso et al., 2014)

Additional Information

Statement

Reference

Marker for

Reflects expression of

ple

(Aso et al., 2014)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R52H03

(Takemura et al., 2017)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R52H03, shi^1.UAS

(Vogt et al., 2014)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R52H03, TrpA1^UAS.(B).cKa

(Vogt et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using shi^1.UAS at 30-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.ple and Hsap\RELA^AD.R52H03 in adults, only during aversive (CS+) odor re-exposure following training, does not prevent the aversive olfactory memory extinction that results from re-exposure, compared to controls.

(Felsenberg et al., 2018)

Adult females prefer optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.ple and Hsap\RELA^AD.R52H03 to darkness with no activation; this preference is significantly greater than that of controls for red light compared to darkness.

(Takemura et al., 2017)

Inhibition (using shi^1.UAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.ple and Hsap\RELA^AD.R52H03 in adults significantly reduces aversive, but not appetitive, 1-min visual memory, compared to controls; inhibition does not significantly affect shock preference, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.ple and Hsap\RELA^AD.R52H03, in combination with a paired light stimulus, results in a significant increase in short-term associative aversive visual memory, compared to controls.

(Vogt et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

70197

w¹¹¹⁸; P{ple-GAL4.DBD}attP2

602073

w¹¹¹⁸; PBac{ple-GAL4.DBD}VK00027

68302

w¹¹¹⁸; P{R30G08-p65.AD}attP40; P{ple-GAL4.DBD}attP2

68329

w¹¹¹⁸; P{R52H03-p65.AD}attP40; P{ple-GAL4.DBD}attP2

Notes on Origin

Discoverer

Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

(Aso et al., 2014)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{DBD.ple.T:Zzzz\Zip-}

Scer\GAL4^DBD.ple

Name Synonyms

Secondary FlyBase IDs

References (41)

Report Sections

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