FlyBase Allele Report: Hsap\RELA[AD.R17A04]

General Information

Symbol

Hsap\RELA^AD.R17A04

Species

H. sapiens

Name

FlyBase ID

FBal0304498

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R17A04-p65.AD}

PBac{R17A04-p65.AD}

Also Known As

R17A04_p65ADZp

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{R17A04-p65.AD}

PBac{R17A04-p65.AD}

Cytology

Description

The R17A04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR17A04

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

descending neuron of the posterior brain DNp28

Comment: when combined with P{R24A03-GAL4.DBD} (combination referred to as 'SS01056')

(Namiki et al., 2022, Namiki et al., 2018)

mushroom body calyx arborizing neuron 1 | intense

Comment: when combined with P{R65D07-GAL4.DBD} (combination referred to as 'MB380B')

(Aso et al., 2014)

lobula columnar neuron LC20

Comment: when combined with P{R35B06-GAL4.DBD} (combination referred to as 'SS00343')

(Wu et al., 2016)

adult ventral nerve cord | restricted

Comment: when combined with P{R35B06-GAL4.DBD} (combination referred to as 'SS00343')

(Wu et al., 2016)

giant fiber neuron

Comment: when combined with P{R68A06-GAL4.DBD}

(von Reyn et al., 2014)

mushroom body calyx arborizing neuron 1

Comment: when combined with P{R65D07-GAL4.DBD} (combination referred to as 'LH2449')

when combined with P{VT006903-GAL4.DBD} (combination referred to as 'LH1950')

when combined with P{VT020605-GAL4.DBD} (combination referred to as 'LH1995')

when combined with P{VT026759-GAL4.DBD} (combination referred to as 'LH1979')

when combined with P{VT041432-GAL4.DBD} (combination referred to as 'LH1900')

(Dolan et al., 2019)

GABAergic neuron | subset

Comment: when combined with P{VT041432-GAL4.DBD} (combination referred to as 'LH1900')

(Tison et al., 2020)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal jump response | adult stage, with Hsap\KCNJ2^{IVS.10xUAS.EGFP}, Scer\GAL4^DBD.R68A06

(von Reyn et al., 2017)

abnormal jump response | adult stage, with Hsap\KCNJ2^{IVS.10xUAS.EGFP}, Scer\GAL4^DBD.R79H02

(von Reyn et al., 2017)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R68A06

(von Reyn et al., 2017)

Phenotype Manifest In

giant fiber neuron | adult stage, with Lis-1^HMS01457, Scer\GAL4^DBD.R68A06

(Kudumala et al., 2017)

transport vesicle | adult stage, with Lis-1^HMS01457, Scer\GAL4^DBD.R68A06

(Kudumala et al., 2017)

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 590 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R68A06 and Hsap\RELA^AD.R17A04 in adults significantly increases takeoff rates, compared to controls; takeoffs are shorter in duration than spontaneous takeoffs in controls.

Silencing (using Hsap\KCNJ2^{IVS.10xUAS.EGFP}) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R68A06 and Hsap\RELA^AD.R17A04 in adults mildly reduces overall takeoff rates in response to looming stimuli, and significantly and substantially reduces the proportion of short takeoffs, compared to controls.

(von Reyn et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions