FB2025_01 , released February 20, 2025
Allele: Hsap\RELAAD.ple
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General Information
Symbol
Hsap\RELAAD.ple
Species
H. sapiens
Name
FlyBase ID
FBal0304536
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{ple-p65.AD}
PBac{ple-p65.AD}
Also Known As
TH-p65ADZp
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Aso et al., 2014)
Progenitor genotype
Carried in construct
P{ple-p65.AD}
PBac{ple-p65.AD}
Cytology
Description

The ple promoter region is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Allele components
Component
Use(s)
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval mushroom body dopaminergic neuron c1

      Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

      when combined with P{R72G06-GAL4.DBD} (combination referred to as 'MB586B')

      (Truman et al., 2023, Eschbach et al., 2020)
      larval mushroom body dopaminergic neuron f1

      Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

      (Truman et al., 2023, Eschbach et al., 2020)
      adult stage
      MB-alpha neuron of the dopaminergic PPL1 cluster

      Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

      (Zhang et al., 2022, Aso and Rubin, 2016, Aso et al., 2014)
      MB-alpha' neuron of the dopaminergic PPL1 cluster | faint

      Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

      (Aso et al., 2014)
      mushroom body medial-vertical lobe arborizing neuron 1

      Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

      when combined with P{R73F07-GAL4.DBD} (combination referred to as 'MB099C')

      (Zhang et al., 2022, Aso and Rubin, 2016, Aso et al., 2014)
      mushroom body vertical lobe arborizing neuron 1

      Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

      when combined with P{R73F07-GAL4.DBD} (combination referred to as 'MB099C')

      (Zhang et al., 2022, Aso and Rubin, 2016, Aso et al., 2014)
      MB-alpha neuron of the dopaminergic PPL1 cluster | faint

      Comment: when combined with P{R26F01-GAL4.DBD} (combination referred to as 'MB308B')

      (Aso et al., 2014)
      MB-alpha' neuron of the dopaminergic PPL1 cluster

      Comment: when combined with P{R26F01-GAL4.DBD} (combination referred to as 'MB308B')

      when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

      (Zhang et al., 2022, Aso and Rubin, 2016, Aso et al., 2014)
      MB-alpha' neuron of the dopaminergic PPL1 cluster | intense

      Comment: when combined with P{R26F01-GAL4.DBD} (combination referred to as 'MB308B')

      (Aso et al., 2014)
      mushroom body pedunculus-medial lobe arborizing neuron 1

      Comment: when combined with P{R22B12-GAL4.DBD} (combination referred to as 'MB320C')

      (Zhang et al., 2022, Feng et al., 2021, Aso and Rubin, 2016)
      dopaminergic PPL2ab neuron

      Comment: when combined with P{R42H01-GAL4.DBD} (combination referred to as 'LH2454')

      (Dolan et al., 2019)
      EB neuron of the dopaminergic PPM3 cluster

      Comment: when combined with P{Ddc-GAL4.DBD}

      (Gao et al., 2019)
      adult dopaminergic VUM neuron of the gnathal ganglion

      Comment: when combined with P{VT064569-GAL4.DBD} (combination referred to as 'SS46889')

      (Sterne et al., 2021)
      adult ascending neuron | subset

      Comment: when combined with P{VT064569-GAL4.DBD} (combination referred to as 'SS46889')

      (Sterne et al., 2021)
      adult dopaminergic PAM neuron | subset

      Comment: when combined with P{VT064569-GAL4.DBD} (combination referred to as 'SS46889')

      (Sterne et al., 2021)
      dopaminergic PPL1 fan-shaped body layer 5 neuron

      Comment: when combined with P{VT025720-GAL4.DBD} (combination referred to as 'SS56699')

      (Hulse et al., 2021)
      dopaminergic PPL1 fan-shaped body layer 6 neuron

      Comment: when combined with P{VT025720-GAL4.DBD} (combination referred to as 'SS56699')

      (Hulse et al., 2021)
      dopaminergic PPL1 fan-shaped body layer 7 neuron

      Comment: when combined with P{VT025720-GAL4.DBD} (combination referred to as 'SS56699')

      (Hulse et al., 2021)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      ple
      (Aso and Rubin, 2016)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult females prefer optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R72B05 and Hsap\RELAAD.ple to darkness with no activation; this preference is significantly greater than that of controls for red light compared to darkness.

      (Takemura et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (10)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Aso et al., 2014)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.ple.T:Zzzz\Zip+
      Hsap\RELAAD.ple
      Name Synonyms
      Secondary FlyBase IDs
        References (41)